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Literature summary for 1.14.20.14 extracted from
- Discovery of a promiscuous non-heme iron halogenase in ambiguine alkaloid biogenesis implication for an evolvable enzyme family for late-stage halogenation of aliphatic carbons in small molecules (2016), Angew. Chem. Int. Ed. Engl., 55, 5780-5784 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Fischerella ambigua |
- |
Hapalosiphon welwitschii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a pair of chimeric proteins by fusing the N-terminal WelO5 (aa1-145) from Hapalosiphon welwitschii UTEX B 1830 with C-terminal AmbO5 (aa146-290) from Fischerella ambigua to give WelO5AmbO5 and vice versa to give AmbO5WelO5. WelO5AmbO5 is able to convert ambiguine C to ambiguine B, albeit at a lower efficiency comparing with wild-type AmbO5, whereas AmbO5WelO5 shows no measurable activity towards ambiguine C under identical assay conditions. This result implicates the C-terminal residues of AmbO5 may play a role on its broadened substrate scope | Fischerella ambigua |
| additional information | generation of a pair of chimeric proteins by fusing the N-terminal WelO5 (aa1-145) from Hapalosiphon welwitschii UTEX B 1830 with C-terminal AmbO5 (aa146-290) from Fischerella ambigua to give WelO5AmbO5 and vice versa to give AmbO5WelO5. WelO5AmbO5 is able to convert ambiguine C to ambiguine B, albeit at a lower efficiency comparing with wild-type AmbO5, whereas AmbO5WelO5 shows no measurable activity towards ambiguine C under identical assay conditions. This result implicates the C-terminal residues of AmbO5 may play a role on its broadened substrate scope | Hapalosiphon welwitschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fischerella ambigua | A0A076NA91 | - |
- |
| Hapalosiphon welwitschii | A0A067YX61 | - |
- |
| Hapalosiphon welwitschii UTEX B 1830 | A0A067YX61 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fischerella ambigua |
- |
Hapalosiphon welwitschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? |  |
| 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
| 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii UTEX B 1830 | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
| 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
| 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
| 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii UTEX B 1830 | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
| ambiguine C + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine B + succinate + CO2 + H2O | - |
? | |
| ambiguine H + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine A + succinate + CO2 + H2O | - |
? | |
| ambiguine I + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine E + succinate + CO2 + H2O | - |
? | |
| ambiguine L + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine K + succinate + CO2 + H2O | - |
? | |
| hapalindole U + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | hapalindole G + succinate + CO2 + H2O | - |
? | |
| additional information | no activity with hapalindole U, ambiguine H, ambiguine C, ambiguine L, ambiguine I, ambiguine E | Hapalosiphon welwitschii | ? | - |
? | |
| additional information | no activity with hapalindole U, ambiguine H, ambiguine C, ambiguine L, ambiguine I, ambiguine E | Hapalosiphon welwitschii UTEX B 1830 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AmbO5 protein | - |
Fischerella ambigua |
| WelO5 protein | - |
Hapalosiphon welwitschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
hapalindole U | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.67 | - |
ambiguine L | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.68 | - |
ambiguine H | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.68 | - |
ambiguine I | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.7 | - |
ambiguine C | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.71 | - |
12-epi-hapalindole C | pH and temperature not specified in the publication | Fischerella ambigua | |
| 0.73 | - |
12-epi-hapalindole C | pH and temperature not specified in the publication | Hapalosiphon welwitschii | |
| 1.7 | - |
12-epi-fischerindole U | pH and temperature not specified in the publication | Fischerella ambigua | |
| 1.8 | - |
12-epi-fischerindole U | pH and temperature not specified in the publication | Hapalosiphon welwitschii | |
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Release 2023.1 (January 2023)
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