BRENDA - Enzyme Database
show all sequences of 1.14.20.13

Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus

Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; He, H.X.; Planta Med. 71, 249-253 (2005)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
in absence of ascorbate, 14% of the activity in presence of 4 mM
Anisodus tanguticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, 100% inhibition
Anisodus tanguticus
Cu2+
5 mM, 100% inhibition
Anisodus tanguticus
EDTA
5 mM, 61% inhibition
Anisodus tanguticus
Fe3+
5 mM, 65% inhibition
Anisodus tanguticus
Mg2+
5 mM, 14% inhibition
Anisodus tanguticus
Ni2+
5 mM, 64% inhibition
Anisodus tanguticus
Zn2+
5 mM, 66% inhibition
Anisodus tanguticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
(6S)-6beta-hydroxyhyoscyamine
pH 7.4, 30°C
Anisodus tanguticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required, only 1.3% of maximum activity in absence of Fe2+
Anisodus tanguticus
Organism
Organism
UniProt
Commentary
Textmining
Anisodus tanguticus
Q6V0J1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
hairy root
Anisodus tanguticus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
746164
Anisodus tanguticus
scopolamine + succinate + CO2 + H2O
-
-
-
?
additional information
enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine
746164
Anisodus tanguticus
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Anisodus tanguticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Anisodus tanguticus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
-
20% of maximum activity
Anisodus tanguticus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
in absence of ascorbate, 14% of the activity in presence of 4 mM
Anisodus tanguticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
5 mM, 100% inhibition
Anisodus tanguticus
Cu2+
5 mM, 100% inhibition
Anisodus tanguticus
EDTA
5 mM, 61% inhibition
Anisodus tanguticus
Fe3+
5 mM, 65% inhibition
Anisodus tanguticus
Mg2+
5 mM, 14% inhibition
Anisodus tanguticus
Ni2+
5 mM, 64% inhibition
Anisodus tanguticus
Zn2+
5 mM, 66% inhibition
Anisodus tanguticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
(6S)-6beta-hydroxyhyoscyamine
pH 7.4, 30°C
Anisodus tanguticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required, only 1.3% of maximum activity in absence of Fe2+
Anisodus tanguticus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
hairy root
Anisodus tanguticus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
746164
Anisodus tanguticus
scopolamine + succinate + CO2 + H2O
-
-
-
?
additional information
enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine
746164
Anisodus tanguticus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Anisodus tanguticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Anisodus tanguticus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
-
20% of maximum activity
Anisodus tanguticus
Other publictions for EC 1.14.20.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744250
Lan
Comparison of two hyoscyamine ...
Anisodus luridus, Hyoscyamus niger
Biochem. Biophys. Res. Commun.
497
25-31
2018
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2
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2
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2
2
-
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-
745181
Ushimaru
Substrate conformation correl ...
Hyoscyamus niger
J. Am. Chem. Soc.
140
7433-7436
2018
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1
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1
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1
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2
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1
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1
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2
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742949
Cao
Efficient biosynthesis of rar ...
Anisodus acutangulus
J. Biotechnol.
211
123-129
2015
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1
1
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1
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1
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1
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1
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724556
Li
Functional characterization of ...
Atropa belladonna
Bioorg. Med. Chem.
20
4356-4363
2012
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1
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8
1
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2
2
-
1
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1
1
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2
7
1
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1
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1
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1
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9
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1
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2
2
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1
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2
7
1
1
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1
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1
1
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725946
Kai
Enhancing the production of tr ...
Anisodus acutangulus
Mol. Biosyst.
8
2883-2890
2012
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2
2
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2
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2
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2
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2
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2
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4
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2
2
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2
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2
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2
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2
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2
2
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726202
Vakili
Chromium-induced tropane alkal ...
Atropa belladonna
Plant Physiol. Biochem.
52
98-103
2012
1
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1
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1
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1
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2
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2
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1
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1
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2
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2
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1
3
3
1
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-
724637
Kai
Functional identification of h ...
Anisodus acutangulus
Biotechnol. Lett.
33
1361-1365
2011
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1
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2
1
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1
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1
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1
1
2
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1
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2
1
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1
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1
1
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1
1
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-
746130
Pramod
Biochemical and structural ch ...
Datura metel
Plant Physiol. Biochem.
48
966-970
2010
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1
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1
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2
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745245
Kai
Molecular cloning and charact ...
Anisodus acutangulus
J. Biochem. Mol. Biol.
40
715-722
2007
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1
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6
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4
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1
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1
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1
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4
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1
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1
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-
744570
Zarate
Tailoring tropane alkaloid ac ...
Hyoscyamus niger
Biotechnol. Lett.
28
1271-1277
2006
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1
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9
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1
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1
1
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-
745463
Haekkinen
Enhanced secretion of tropane ...
Hyoscyamus niger
J. Exp. Bot.
56
2611-2618
2005
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8
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1
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746164
Liu
Molecular cloning, expression ...
Anisodus tanguticus
Planta Med.
71
249-253
2005
1
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7
1
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1
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5
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1
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2
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1
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1
1
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1
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7
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1
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1
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1
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2
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1
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1
1
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746087
Suzuki
An Atropa belladonna hyoscyam ...
Atropa belladonna
Plant Mol. Biol.
40
141-152
1999
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7
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7
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7
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-
439268
Hashimoto
-
6beta-hydroxyhyoscyamine epoxi ...
Hyoscyamus niger
Phytochemistry
28
1077-1082
1989
1
-
-
-
-
1
6
1
1
1
-
1
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1
1
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1
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1
1
1
2
-
1
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1
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2
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1
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2
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1
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6
-
1
1
1
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1
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1
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1
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1
1
1
2
-
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1
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-
744828
Hashimoto
Purification and characteriza ...
Hyoscyamus niger
Eur. J. Biochem.
164
277-285
1987
4
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-
12
2
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1
1
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1
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1
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1
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1
9
-
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1
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2
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4
-
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12
2
2
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1
1
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1
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1
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1
9
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1
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