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Literature summary for 1.14.20.13 extracted from

  • Hashimoto, T.; Yamada, Y.
    Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. hydroxylase and epoxidase activities in the enzyme preparation (1987), Eur. J. Biochem., 164, 277-285 .
    View publication on PubMed
Search for more literature for 1.14.20.13

Activating Compound

Activating Compound Comment Organism Structure
ascorbate presence of reductant required Hyoscyamus niger
dehydroascorbate
-
 Hyoscyamus niger
dithiothreitol
-
 Hyoscyamus niger
Fe2+
-
 Hyoscyamus niger

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxybenzoate competitive inhibitor with respect to 2-oxoglutarate Hyoscyamus niger
Cd2+ 0.4 mM, 100% inhibition Hyoscyamus niger
Co2+ 0.4 mM, 100% inhibition Hyoscyamus niger
Cu2+ 0.4 mM, 100% inhibition Hyoscyamus niger
EDTA 0.1 mM, 67% inhibition Hyoscyamus niger
Hg2+ 0.4 mM, 96% inhibition Hyoscyamus niger
Mn2+ 0.4 mM, 95% inhibition Hyoscyamus niger
Ni2+ 0.4 mM, 96% inhibition Hyoscyamus niger
nitroblue tetrazolium
-
 Hyoscyamus niger
Pyridine 2,4-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate Hyoscyamus niger
Tiron
-
 Hyoscyamus niger
Zn2+ 0.4 mM, 100% inhibition Hyoscyamus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 (6S)-6beta-hydroxyhyoscyamine pH 7.8, 30°C Hyoscyamus niger
0.043
-
 2-oxoglutarate pH 7.8, 30°C Hyoscyamus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel fitlration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 Hyoscyamus niger
-
 scopolamine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Hyoscyamus niger P24397
-
-

Purification (Commentary)

Purification (Comment) Organism
from root culture Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
root root culture Hyoscyamus niger
-

Storage Stability

Storage Stability Organism
-20°C, stable for at least 3 months Hyoscyamus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger scopolamine + succinate + CO2 + H2O
-
 ?
3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 + H2O
-
 ?
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger scopolamine + succinate + CO2
-
 ?
apoatropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyapoatropine + succinate + CO2 + H2O
-
 ?
l-homatropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyhomatropine + succinate + CO2 + H2O
-
 ?
additional information enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine Hyoscyamus niger ?
-
 ?
norhyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxynorhyoscyamine + succinate + CO2 + H2O
-
 ?
phenylacetyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6-hydroxyphenylacetyltropine + succinate + CO2 + H2O
-
 ?
t-cinnamoyltropine + 2-oxoglutarate + O2
-
 Hyoscyamus niger t-cinnamoyl-6-hydroxytropine + succinate + CO2 + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Hyoscyamus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
 Pyridine 2,4-dicarboxylate pH 7.8, 30°C Hyoscyamus niger
0.09
-
 3,4-dihydroxybenzoate pH 7.8, 30°C Hyoscyamus niger