Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3) | Anisodus acutangulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
64000 | - |
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
scopolamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anisodus acutangulus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His- or GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel or glutathione affinity chromatography, respectively | Anisodus acutangulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | scopolamine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
Synonyms | Comment | Organism |
---|---|---|
H6H | - |
Anisodus acutangulus |
hyoscyamine 6beta-hydroxylase | - |
Anisodus acutangulus |
General Information | Comment | Organism |
---|---|---|
metabolism | hyoscyamine 6beta-hydroxylase converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway | Anisodus acutangulus |