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Literature summary for 1.14.20.13 extracted from

  • Kai, G.; Liu, Y.; Wang, X.; Yang, S.; Fu, X.; Luo, X.; Liao, P.
    Functional identification of hyoscyamine 6beta-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli (2011), Biotechnol. Lett., 33, 1361-1365.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Anisodus acutangulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus
64000
-
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 Anisodus acutangulus
-
scopolamine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Anisodus acutangulus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His- or GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel or glutathione affinity chromatography, respectively Anisodus acutangulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
Anisodus acutangulus scopolamine + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus

Synonyms

Synonyms Comment Organism
H6H
-
Anisodus acutangulus
hyoscyamine 6beta-hydroxylase
-
Anisodus acutangulus

General Information

General Information Comment Organism
metabolism hyoscyamine 6beta-hydroxylase converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway Anisodus acutangulus