BRENDA - Enzyme Database show
show all sequences of 1.14.20.13

6beta-hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger

Hashimoto, T.; Kohno, J.; Yamada, Y.; Phytochemistry 28, 1077-1082 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
catalase
-
Hyoscyamus niger
General Stability
General Stability
Organism
rapid loss of enzyme activity during purification
Hyoscyamus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
100% inhibition
Hyoscyamus niger
MgSO4
24% inhibition
Hyoscyamus niger
MnSO4
100% inhibition
Hyoscyamus niger
additional information
no marked effect on enzyme activity by addition of NAD+, NADH, NADP+, NADPH, ATP + MgSO4, FAD, FMN, pyrroloquinoline quinone, acetyl-CoA, 6,7-dimethyl-5,6,7,8-tetrahydrofolate, phenazine methosulfate, 2,6-dichlorophenolindophenol, cytochrome c and H2O2
Hyoscyamus niger
Pyridine 2,4-dicarboxylate
-
Hyoscyamus niger
ZnSO4
100% inhibition
Hyoscyamus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
(6S)-6-hydroxyhyoscyamine
-
Hyoscyamus niger
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Hyoscyamus niger
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required as co-factor
Hyoscyamus niger
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
Hyoscyamus niger
alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species
scopolamine + succinate + CO2
-
Hyoscyamus niger
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyoscyamus niger
-
-
-
Oxidation Stability
Oxidation Stability
Organism
requires molecular oxygen
Hyoscyamus niger
Purification (Commentary)
Commentary
Organism
partial, could not be separated from hyoscyamine 6beta-hydroxylase
Hyoscyamus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Hyoscyamus niger
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000198
-
-
Hyoscyamus niger
Storage Stability
Storage Stability
Organism
-20°C, enzyme is stored in small portions until assayed
Hyoscyamus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
439268
Hyoscyamus niger
scopolamine + succinate + CO2
-
439268
Hyoscyamus niger
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species
439268
Hyoscyamus niger
scopolamine + succinate + CO2
-
439268
Hyoscyamus niger
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hyoscyamus niger
Cofactor
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
-
Hyoscyamus niger
ascorbate
-
Hyoscyamus niger
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
catalase
-
Hyoscyamus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
-
Hyoscyamus niger
ascorbate
-
Hyoscyamus niger
General Stability (protein specific)
General Stability
Organism
rapid loss of enzyme activity during purification
Hyoscyamus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
100% inhibition
Hyoscyamus niger
MgSO4
24% inhibition
Hyoscyamus niger
MnSO4
100% inhibition
Hyoscyamus niger
additional information
no marked effect on enzyme activity by addition of NAD+, NADH, NADP+, NADPH, ATP + MgSO4, FAD, FMN, pyrroloquinoline quinone, acetyl-CoA, 6,7-dimethyl-5,6,7,8-tetrahydrofolate, phenazine methosulfate, 2,6-dichlorophenolindophenol, cytochrome c and H2O2
Hyoscyamus niger
Pyridine 2,4-dicarboxylate
-
Hyoscyamus niger
ZnSO4
100% inhibition
Hyoscyamus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
(6S)-6-hydroxyhyoscyamine
-
Hyoscyamus niger
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Hyoscyamus niger
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required as co-factor
Hyoscyamus niger
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
Hyoscyamus niger
alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species
scopolamine + succinate + CO2
-
Hyoscyamus niger
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
requires molecular oxygen
Hyoscyamus niger
Purification (Commentary) (protein specific)
Commentary
Organism
partial, could not be separated from hyoscyamine 6beta-hydroxylase
Hyoscyamus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Hyoscyamus niger
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000198
-
-
Hyoscyamus niger
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, enzyme is stored in small portions until assayed
Hyoscyamus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
439268
Hyoscyamus niger
scopolamine + succinate + CO2
-
439268
Hyoscyamus niger
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species
439268
Hyoscyamus niger
scopolamine + succinate + CO2
-
439268
Hyoscyamus niger
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hyoscyamus niger
Other publictions for EC 1.14.20.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744250
Lan
Comparison of two hyoscyamine ...
Anisodus luridus, Hyoscyamus niger
Biochem. Biophys. Res. Commun.
497
25-31
2018
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2
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2
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2
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2
2
-
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-
745181
Ushimaru
Substrate conformation correl ...
Hyoscyamus niger
J. Am. Chem. Soc.
140
7433-7436
2018
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1
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1
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1
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2
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1
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1
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2
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742949
Cao
Efficient biosynthesis of rar ...
Anisodus acutangulus
J. Biotechnol.
211
123-129
2015
-
1
1
-
1
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1
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1
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1
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1
1
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1
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1
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1
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724556
Li
Functional characterization of ...
Atropa belladonna
Bioorg. Med. Chem.
20
4356-4363
2012
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-
1
-
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-
8
1
-
-
2
2
-
1
-
-
1
1
-
-
-
2
7
1
1
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-
1
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-
1
-
-
-
-
-
8
-
1
-
-
2
2
-
-
-
1
-
-
-
2
7
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
725946
Kai
Enhancing the production of tr ...
Anisodus acutangulus
Mol. Biosyst.
8
2883-2890
2012
-
1
1
-
1
-
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2
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2
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1
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2
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2
2
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2
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2
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2
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2
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1
2
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-
726202
Vakili
Chromium-induced tropane alkal ...
Atropa belladonna
Plant Physiol. Biochem.
52
98-103
2012
1
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1
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1
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1
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1
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1
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1
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2
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2
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1
3
3
1
-
-
724637
Kai
Functional identification of h ...
Anisodus acutangulus
Biotechnol. Lett.
33
1361-1365
2011
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1
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2
1
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1
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1
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1
1
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1
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2
1
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1
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1
1
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1
1
-
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-
746130
Pramod
Biochemical and structural ch ...
Datura metel
Plant Physiol. Biochem.
48
966-970
2010
-
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1
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1
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2
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1
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2
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745245
Kai
Molecular cloning and charact ...
Anisodus acutangulus
J. Biochem. Mol. Biol.
40
715-722
2007
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1
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5
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4
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1
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1
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1
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4
-
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1
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1
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-
744570
Zarate
Tailoring tropane alkaloid ac ...
Hyoscyamus niger
Biotechnol. Lett.
28
1271-1277
2006
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1
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5
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1
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1
1
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745463
Haekkinen
Enhanced secretion of tropane ...
Hyoscyamus niger
J. Exp. Bot.
56
2611-2618
2005
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6
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1
1
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746164
Liu
Molecular cloning, expression ...
Anisodus tanguticus
Planta Med.
71
249-253
2005
1
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7
1
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1
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4
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1
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2
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1
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1
1
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7
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1
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1
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1
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2
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1
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1
1
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746087
Suzuki
An Atropa belladonna hyoscyam ...
Atropa belladonna
Plant Mol. Biol.
40
141-152
1999
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5
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5
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5
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439268
Hashimoto
-
6beta-hydroxyhyoscyamine epoxi ...
Hyoscyamus niger
Phytochemistry
28
1077-1082
1989
1
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-
1
6
1
1
1
-
1
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1
1
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1
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-
1
1
1
2
-
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-
-
1
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2
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1
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2
-
-
1
-
6
-
1
1
1
-
1
-
1
-
1
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1
1
1
2
-
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1
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744828
Hashimoto
Purification and characteriza ...
Hyoscyamus niger
Eur. J. Biochem.
164
277-285
1987
4
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12
2
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1
1
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1
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1
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1
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1
9
-
-
-
-
-
1
-
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-
2
-
-
4
-
-
-
-
-
-
-
12
2
2
-
-
1
1
-
-
-
1
-
1
-
1
9
-
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-
-
-
1
-
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-
-
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