Protein Variants | Comment | Organism |
---|---|---|
C155Y | site-directed mutagenesis | Streptomyces clavuligerus |
C155Y/Y184H/V275I/C281Y | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
C281Y | site-directed mutagenesis | Streptomyces clavuligerus |
I305L | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
I305M | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
I305M/S261M | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
I305M/T213V | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
I305M/T213V/M73T | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
I305M/T213V/S261M | site-directed mutagenesis, the mutant shows increased activity with penicillin G compared to the wild-type enzyme | Streptomyces clavuligerus |
M73T | site-directed mutagenesis | Streptomyces clavuligerus |
additional information | since the natural substrate of the enzyme, penicillin N, is not available in large quantities, engineering of a scDAOCS capable of converting the cheap and easily available substrate penicillin G is valuable for large-scale production of 7-aminodeacetoxycephalosporanic acid, 7-ADCA | Streptomyces clavuligerus |
N304K | site-directed mutagenesis | Streptomyces clavuligerus |
Q126M | site-directed mutagenesis | Streptomyces clavuligerus |
S261A | site-directed mutagenesis | Streptomyces clavuligerus |
S261M | site-directed mutagenesis | Streptomyces clavuligerus |
T213V | site-directed mutagenesis | Streptomyces clavuligerus |
V275I | site-directed mutagenesis | Streptomyces clavuligerus |
Y184H | site-directed mutagenesis | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
penicillin G | mutant C155Y/Y184H/V275I/C281Y, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
1.62 | - |
penicillin G | mutant I305M/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
1.92 | - |
penicillin G | mutantI305M/T213V/M73T, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
2.1 | - |
penicillin G | mutant I305L, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
2.49 | - |
penicillin G | mutant I305M/T213V/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
3.22 | - |
penicillin G | mutant I305M/T213V, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
3.58 | - |
penicillin G | mutant I305M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
14.38 | - |
penicillin G | wild-type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | Streptomyces clavuligerus | the enzyme catalyzes the ring expansion of penicillin substrates into a ring-expanded product to produce cephamycin C | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | the enzyme catalyzes the ring expansion of penicillin substrates into a ring-expanded product to produce cephamycin C | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAOCS | - |
Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
penicillin G | wild-type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.23 | - |
penicillin G | mutant C155Y/Y184H/V275I/C281Y, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.37 | - |
penicillin G | mutant I305L, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.537 | - |
penicillin G | mutant I305M/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.589 | - |
penicillin G | mutantI305M/T213V/M73T, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.65 | - |
penicillin G | mutant I305M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.654 | - |
penicillin G | mutant I305M/T213V, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.668 | - |
penicillin G | mutant I305M/T213V/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
penicillin G | wild-type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.144 | - |
penicillin G | mutant C155Y/Y184H/V275I/C281Y, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.176 | - |
penicillin G | mutant I305L, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.182 | - |
penicillin G | mutant I305M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.203 | - |
penicillin G | mutant I305M/T213V, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.268 | - |
penicillin G | mutant I305M/T213V/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.307 | - |
penicillin G | mutantI305M/T213V/M73T, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.332 | - |
penicillin G | mutant I305M/S261M, pH and temperature not specified in the publication | Streptomyces clavuligerus |