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Literature summary for 1.14.20.1 extracted from

  • Baldwin, J.E.; Blackburn, J.M.; Heath, R.J.; Sutherland, J.D.
    High-level soluble expression and purification of deacetoxycephalosporin C/deacetylcephalosporin C synthase (1992), Bioorg. Med. Chem. Lett., 2, 663-668.
No PubMed abstract available
Search for more literature for 1.14.20.1

Cloned(Commentary)

Cloned (Comment) Organism
expression at high levels in Escherichia coli under control of the trc promoter Acremonium chrysogenum

General Stability

General Stability Organism
purified enzyme is stable when flash-frozen and stored at -80°C Acremonium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Acremonium chrysogenum
-
-

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acremonium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acremonium chrysogenum

Storage Stability

Storage Stability Organism
purified enzyme is stable when flash-frozen and stored at -80°C Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin N + 2-oxoglutarate + O2
-
 Acremonium chrysogenum deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?