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Literature summary for 1.14.20.1 extracted from

  • Chin, H.S.; Sim, J.; Seah, K.I.; Sim, T.S.
    Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity (2003), FEMS Microbiol. Lett., 218, 251-257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) Streptomyces clavuligerus
DNA and amino acid sequence determination and analysis, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) Streptomyces jumonjinensis
DNA and amino acid sequence determination and analysis, expression of the wild-type enzyme and a C-terminally truncated mutant enzyme lacking 20 amino acids as GST-fusion proteins in Escherichia coli strain BL21(DE3) Acremonium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron dependent on Streptomyces clavuligerus
Iron dependent on Streptomyces jumonjinensis
Iron dependent on Acremonium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Streptomyces clavuligerus
60000
-
x * 60000, SDS-PAGE Streptomyces jumonjinensis
60000
-
x * 60000, recombinant enzyme, SDS-PAGE Streptomyces clavuligerus
60000
-
x * 60000, recombinant enzyme, SDS-PAGE Streptomyces jumonjinensis
60000
-
x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme Acremonium chrysogenum
62000
-
x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deacetoxycephalosporin C + 2-oxoglutarate + O2 Acremonium chrysogenum
-
deacetylcephalosporin C + succinate + CO2
-
ir
additional information Acremonium chrysogenum the enzyme catalyzes the first and second step in the cephamycin C biosynthetic pathway ?
-
?
penicillin N + 2-oxoglutarate + O2 Acremonium chrysogenum
-
deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2 Streptomyces clavuligerus first step in cephamycin C biosynthetic pathway deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2 Streptomyces jumonjinensis first step in cephamycin C biosynthetic pathway deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
-
-
Acremonium chrysogenum Q9P4T5 bifunctional enzyme
-
Streptomyces clavuligerus
-
-
-
Streptomyces jumonjinensis
-
-
-
Streptomyces jumonjinensis Q93FD4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Streptomyces clavuligerus
recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Streptomyces jumonjinensis
recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Acremonium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
recombinant enzyme, substrate amoxicillin Streptomyces jumonjinensis
0.014
-
purified recombinant wild-type and truncated mutant enzyme, substrate metampicillin Acremonium chrysogenum
0.015
-
recombinant enzyme, substrate metampicillin Streptomyces clavuligerus
0.023
-
purified recombinant truncated mutant enzyme, substrate carbenicillin Acremonium chrysogenum
0.026
-
purified recombinant wild-type enzyme, substrate carbenicillin Acremonium chrysogenum
0.026
-
recombinant enzyme, substrate metampicillin Streptomyces jumonjinensis
0.037
-
recombinant enzyme, substrate carbenicillin Streptomyces clavuligerus
0.039
-
purified recombinant wild-type and truncated mutant enzyme, substrate ampicillin, determined by bioassay Acremonium chrysogenum
0.051
-
recombinant enzyme, substrate penicillin V Streptomyces jumonjinensis
0.071
-
purified recombinant truncated mutant enzyme, substrate phenethicillin Acremonium chrysogenum
0.074
-
purified recombinant wild-type enzyme, substrate phenethicillin Acremonium chrysogenum
0.128
-
recombinant enzyme, substrate carbenicillin Streptomyces jumonjinensis
0.133
-
purified recombinant wild-type enzyme, substrate ampicillin, determined by HPLC Acremonium chrysogenum
0.178
-
purified recombinant truncated mutant enzyme, substrate ampicillin, determined by HPLC Acremonium chrysogenum
0.203
-
recombinant enzyme, substrate ampicillin, determined by bioassay Streptomyces clavuligerus
0.237
-
purified recombinant truncated mutant enzyme, substrate penicillin G Acremonium chrysogenum
0.24
-
purified recombinant wild-type enzyme, substrate penicillin G Acremonium chrysogenum
0.36
-
recombinant enzyme, substrate penicillin G Streptomyces clavuligerus
0.411
-
recombinant enzyme, substrate phenethicillin Streptomyces clavuligerus
0.458
-
recombinant enzyme, substrate ampicillin, determined by bioassay Streptomyces jumonjinensis
0.932
-
recombinant enzyme, substrate ampicillin Streptomyces jumonjinensis
0.932
-
recombinant enzyme, substrate ampicillin, determined by HPLC Streptomyces jumonjinensis
1.042
-
recombinant enzyme, substrate penicillin G Streptomyces jumonjinensis
1.551
-
recombinant enzyme, substrate phenethicillin Streptomyces jumonjinensis
2.221
-
recombinant enzyme, substrate ampicillin, determined by HPLC Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amoxicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ? + succinate + CO2 + H2O
-
?
amoxicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
ampicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus cephalexin + succinate + CO2 + H2O
-
?
ampicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis cephalexin + succinate + CO2 + H2O
-
?
ampicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ?
-
ir
ampicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
ampicillin + 2-oxoglutarate + O2
-
Acremonium chrysogenum ?
-
ir
carbenicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ? + succinate + CO2 + H2O
-
?
carbenicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ? + succinate + CO2 + H2O
-
?
carbenicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ?
-
ir
carbenicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
carbenicillin + 2-oxoglutarate + O2
-
Acremonium chrysogenum ?
-
ir
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
Acremonium chrysogenum deacetylcephalosporin C + succinate + CO2
-
ir
metampicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ? + succinate + CO2 + H2O
-
?
metampicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ? + succinate + CO2 + H2O
-
?
metampicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ?
-
ir
metampicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
metampicillin + 2-oxoglutarate + O2 best substrate Acremonium chrysogenum ?
-
ir
additional information the enzyme catalyzes the first and second step in the cephamycin C biosynthetic pathway Acremonium chrysogenum ?
-
?
additional information substrate specificity, amoxicillin, penicillin V, and oxacillin are no substrate Streptomyces clavuligerus ?
-
?
additional information substrate specificity, oxacillin is no substrate Streptomyces jumonjinensis ?
-
?
additional information substrate specificity, oxacillin, penicillin V, and amoxicillin are no substrate Acremonium chrysogenum ?
-
?
penicillin G + 2-oxoglutarate + O2
-
Streptomyces clavuligerus phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
?
penicillin G + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
?
penicillin G + 2-oxoglutarate + O2
-
Streptomyces clavuligerus phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O
-
ir
penicillin G + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O
-
ir
penicillin G + 2-oxoglutarate + O2
-
Acremonium chrysogenum phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2
-
Acremonium chrysogenum deacetoxycephalosporin C + succinate + CO2 + H2O
-
?
penicillin N + 2-oxoglutarate + O2
-
Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
?
penicillin N + 2-oxoglutarate + O2
-
Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis deacetoxycephalosporin C + succinate + CO2 + H2O
-
?
penicillin N + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2
-
Acremonium chrysogenum deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2 first step in cephamycin C biosynthetic pathway Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin N + 2-oxoglutarate + O2 first step in cephamycin C biosynthetic pathway Streptomyces jumonjinensis deacetoxycephalosporin C + succinate + CO2 + H2O
-
ir
penicillin V + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ? + succinate + CO2 + H2O
-
?
penicillin V + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
phenethicillin + 2-oxoglutarate + O2
-
Streptomyces clavuligerus ? + succinate + CO2 + H2O
-
?
phenethicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ? + succinate + CO2 + H2O
-
?
phenethicillin + 2-oxoglutarate + O2
-
Streptomyces jumonjinensis ?
-
ir
phenethicillin + 2-oxoglutarate + O2
-
Acremonium chrysogenum ?
-
ir
phenethicillin + 2-oxoglutarate + O2 best substrate Streptomyces clavuligerus ?
-
ir

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Streptomyces clavuligerus
? x * 60000, SDS-PAGE Streptomyces jumonjinensis
? x * 60000, recombinant enzyme, SDS-PAGE Streptomyces clavuligerus
? x * 60000, recombinant enzyme, SDS-PAGE Streptomyces jumonjinensis
? x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme Acremonium chrysogenum

Synonyms

Synonyms Comment Organism
DAOC/DACS
-
Acremonium chrysogenum
DAOCS
-
Streptomyces clavuligerus
DAOCS
-
Streptomyces jumonjinensis
deacetoxy/deacetylcephalosporin C synthase
-
Acremonium chrysogenum
deacetoxycephalosporin C synthase
-
Streptomyces clavuligerus
deacetoxycephalosporin C synthase
-
Streptomyces jumonjinensis
expandase
-
Streptomyces clavuligerus
expandase
-
Streptomyces jumonjinensis
expandase
-
Acremonium chrysogenum