Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
bifunctional enzyme deacetoxycephalosporin C synthetase/hydroxylase | - |
Streptomyces clavuligerus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of partially folded recombinant expandase/hydroxylase from insoluble granules of recombinant Escherichia coli | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | separate genes encode penicillin N expandase and DAOC hydroxylase | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | bifunctional enzyme: penicillin N expandase/DAOC hydroxylase | Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |