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Literature summary for 1.14.19.B10 extracted from

  • Neubauer, P.R.; Widmann, C.; Wibberg, D.; Schroeder, L.; Frese, M.; Kottke, T.; Kalinowski, J.; Niemann, H.H.; Sewald, N.
    A flavin-dependent halogenase from metagenomic analysis prefers bromination over chlorination (2018), PLoS ONE, 13, e0196797 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.B10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Brevundimonas sp. BAL3

Crystallization (Commentary)

Crystallization (Comment) Organism
using 1.0 M Na/K tartrate, 0.1 M MES pH 6.0 Brevundimonas sp. BAL3

Organism

Organism UniProt Comment Textmining
Brevundimonas sp. BAL3 B4WBL8
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Talon column chromatography, Ni2+-affinity chromatography, Source 15 Q column chromatography, and Superdex S200 gel filtration Brevundimonas sp. BAL3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + FADH2 + bromide + O2 + H+
-
 Brevundimonas sp. BAL3 3-bromoindole + FAD + 2 H2O
-
 ?
indole + FADH2 + chloride + O2 + H+ low activity Brevundimonas sp. BAL3 3-chloroindole + FAD + 2 H2O
-
 ?
additional information the enzyme prefers bromide to chloride as halide source. The enzyme shows no halogenation of tryptophan, neither in presence of chloride nor in presence of bromide and has no activity with 5-hydroxytryptohan, tryptophol, indole-3-propionic acid, and indole-3-acetonitrile Brevundimonas sp. BAL3 ?
-
-

Synonyms

Synonyms Comment Organism
brvH
-
 Brevundimonas sp. BAL3
FHal
-
 Brevundimonas sp. BAL3
flavin-dependent halogenase
-
 Brevundimonas sp. BAL3

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Brevundimonas sp. BAL3
NAD+
-
 Brevundimonas sp. BAL3