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Literature summary for 1.14.19.74 extracted from

  • Ono, E.; Nakai, M.; Fukui, Y.; Tomimori, N.; Fukuchi-Mizutani, M.; Saito, M.; Satake, H.; Tanaka, T.; Katsuta, M.; Umezawa, T.; Tanaka, Y.
    Formation of two methylenedioxy bridges by a Sesamum CYP81Q protein yielding a furofuran lignan, (+)-sesamin (2006), Proc. Natl. Acad. Sci. USA, 103, 10116-10121 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed heterologously in yeast Sesamum indicum
expressed heterologously in yeast Sesamum radiatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
 (+)-pinoresinol pH 7.4, 30°C Sesamum indicum
0.0117
-
 (+)-piperitol pH 7.4, 30°C Sesamum indicum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum cytoplasmic surface Sesamum indicum 5783
-
endoplasmic reticulum cytoplasmic surface Sesamum radiatum 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum indicum
-
 (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum radiatum
-
 (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum indicum
-
 (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum radiatum
-
 (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sesamum indicum Q33DY0
-
-
Sesamum radiatum Q33DX9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Sesamum indicum
-
additional information the enzyme is not expressed in leaves, stems, seed pods, or flowers Sesamum radiatum
-
additional information the enzyme is not expressed in petals, or stems Sesamum indicum
-
seed
-
 Sesamum radiatum
-
seed expressed substantially in the later phases of seed development and then decreases before seed maturation is complete Sesamum indicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum indicum (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum radiatum (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum indicum (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum radiatum (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information neither (+)-sesamolinol nor (+)-phillygenin is used as substrate Sesamum radiatum ?
-
 ?
additional information the enzyme does not catalyze (+)-sesamolin biosynthesis from (+)-sesamolinol Sesamum indicum ?
-
 ?

Synonyms

Synonyms Comment Organism
(+)-piperitol/(+)-sesamin synthase
-
 Sesamum indicum
(+)-piperitol/(+)-sesamin synthase
-
 Sesamum radiatum
CYP81Q1 protein
-
 Sesamum indicum
CYP81Q2 protein
-
 Sesamum radiatum
PSS
-
 Sesamum indicum
PSS
-
 Sesamum radiatum
SiP189
-
 Sesamum indicum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 a cytochrome P-450 (heme-thiolate) protein Sesamum indicum
cytochrome P-450 a cytochrome P-450 (heme-thiolate) protein Sesamum radiatum

Expression

Organism Comment Expression
Sesamum indicum expressed substantially in the later phases of seed development and then decreases before seed maturation is complete up

General Information

General Information Comment Organism
metabolism the enzyme is involved in furofuran lignan biosynthesis Sesamum indicum
metabolism the enzyme is involved in furofuran lignan biosynthesis Sesamum radiatum