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Literature summary for 1.14.19.74 extracted from

  • Noguchi, A.; Horikawa, M.; Murata, J.; Tera, M.; Kawai, Y.; Ishiguro, M.; Umezawa, T.; Mizutani, M.; Ono, E.
    Mode-of-action and evolution of methylenedioxy bridge forming P450s in plant specialized metabolism (2014), Plant Biotechnol., 31, 493-503 .
No PubMed abstract available
Search for more literature for 1.14.19.74

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of wild-type enzyme and mutant enzymes A308S and A308T in Sf-9 cells Sesamum indicum

Protein Variants

Protein Variants Comment Organism
A308S the mutant enzyme exhibits comparable substrate specificity (Km) to those of wild-type, and its catalytic activity (kcat) is moderately decreased to approximately one-third of wild-type value Sesamum indicum
A308T compared to those of wild-type enzyme, the Km-value is increased to 6fold and the kcat is decreased to approximately 52fold Sesamum indicum

Inhibitors

Inhibitors Comment Organism Structure
(+)-kobusin
-
 Sesamum indicum
(+)-pinoresinol
-
 Sesamum indicum
5-[(1S,3aS,4R,6aR)-4-(1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]-2-methoxyphenol
-
 Sesamum indicum
additional information no inhibition by (+)-epipinoresinol and (-)-pinoresinol Sesamum indicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE Sesamum indicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum indicum
-
 (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2 Sesamum indicum
-
 (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sesamum indicum Q33DY0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum indicum (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sesamum indicum (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information no activity with (+)-phylligenin, (+)-epipinoresinol, (+)-pinoresinol 4'-O-glucoside, (+)-pinoreinol monomethylether, (+)-kobusin, (+)-syringaresinol, (-)-syringaresinol, (-)-lariciresinol, (-)-pinoresinol, (-)-matairesinol or (+)-sesamolinol Sesamum indicum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Sesamum indicum

Synonyms

Synonyms Comment Organism
CYP81Q1
-
 Sesamum indicum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 a cytochrome P-450 (heme-thiolate) protein Sesamum indicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0198
-
 5-[(1S,3aS,4R,6aR)-4-(1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]-2-methoxyphenol pH and temperature not specified in the publication Sesamum indicum