Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
ligand-bound enzyme, sitting drop vapor diffusion method, using in 100 mM Na-MES bufferat pH 5.0-5.5 and 1.6-2.2 M (NH4)2SO4, at 6°C | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one | - |
Mycobacterium tuberculosis | |
cyclo(L-tyrosyl-L-alanyl) | - |
Mycobacterium tuberculosis | |
cyclo(L-tyrosyl-L-phenylalanyl) | - |
Mycobacterium tuberculosis | |
cyclo(L-tyrosyl-L-tryptophanyl) | - |
Mycobacterium tuberculosis | |
cyclo-(L-tyrosyl-DOPA) | - |
Mycobacterium tuberculosis | |
additional information | not inhibited by cyclo(L-Tyr-L-Leu) and cyclo(L-Tyr-L-Met) | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPP7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPP7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | 98% of the compound remains after 1 h incubation with CYP121 | Mycobacterium tuberculosis | ? | - |
? | |
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | 98% of the compound remains after 1 h incubation with CYP121 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
cyclo(L-tyrosyl-L-phenylalanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | the rate of transformation of cyclo(L-tyrosyl-L-phenylalanyl) is very slow, with about 98% of compound remaining after 1 h of incubation with CYP121 | Mycobacterium tuberculosis | ? | - |
? | |
cyclo(L-tyrosyl-L-phenylalanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | the rate of transformation of cyclo(L-tyrosyl-L-phenylalanyl) is very slow, with about 98% of compound remaining after 1 h of incubation with CYP121 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
cyclo(L-tyrosyl-L-tryptophanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | about 50% of the initial compound remains after a 1 h incubation with CYP121 | Mycobacterium tuberculosis | ? | - |
? | |
cyclo(L-tyrosyl-L-tryptophanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | about 50% of the initial compound remains after a 1 h incubation with CYP121 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | 100% transformation | Mycobacterium tuberculosis | mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | 100% transformation | Mycobacterium tuberculosis H37Rv | mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cyclo-(L-tyrosyl-DOPA) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | transformation of cyclo-(L-tyrosyl-DOPA) by CYP121 is very slow, 91% of the compound remains after 1 h of incubation with CYP121 | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with cyclo(L-tyrosyl-L-alanyl) | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with cyclo(L-tyrosyl-L-alanyl) | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP121 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | CYP121 is a highly specific cytochrome P450 | Mycobacterium tuberculosis |