Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain YPL 154C:Pep4 | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00463 | - |
(S)-tetrahydrocolumbamine | in 100 mM HEPES-NaOH, pH 7.5, at 30°C | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine | (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | - |
Papaver somniferum | - |
leaf | - |
Papaver somniferum | - |
root | lowest expression in root | Papaver somniferum | - |
stem | highest expression in stem | Papaver somniferum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 | strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine | Papaver somniferum | (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
canadine synthase | - |
Papaver somniferum |
CYP719A21 | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
malfunction | virus-induced gene silencing of CYP719A21 causes a significant increase in (S)-tetrahydrocolumbamine accumulation and a corresponding decrease in the levels of putative downstream intermediates and noscapine | Papaver somniferum |