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Literature summary for 1.14.19.68 extracted from
- Cloning and characterization of canadine synthase involved in noscapine biosynthesis in opium poppy (2014), FEBS Lett., 588, 198-204.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain YPL 154C:Pep4 | Papaver somniferum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00463 | - |
(S)-tetrahydrocolumbamine | in 100 mM HEPES-NaOH, pH 7.5, at 30°C | Papaver somniferum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine | (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papaver somniferum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | - |
Papaver somniferum | - |
| leaf | - |
Papaver somniferum | - |
| root | lowest expression in root | Papaver somniferum | - |
| stem | highest expression in stem | Papaver somniferum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 | strict substrate specificity and high affinity for (S)-tetrahydrocolumbamine | Papaver somniferum | (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| canadine synthase | - |
Papaver somniferum |
| CYP719A21 | - |
Papaver somniferum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Papaver somniferum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Papaver somniferum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | virus-induced gene silencing of CYP719A21 causes a significant increase in (S)-tetrahydrocolumbamine accumulation and a corresponding decrease in the levels of putative downstream intermediates and noscapine | Papaver somniferum |
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Release 2023.1 (January 2023)
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