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Literature summary for 1.14.19.65 extracted from

  • Hori, K.; Okano, S.; Sato, F.
    Efficient microbial production of stylopine using a Pichia pastoris expression system (2016), Sci. Rep., 6, 22201 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
pharmacology a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica
synthesis a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline) Eschscholzia californica

Organism

Organism UniProt Comment Textmining
Eschscholzia californica B5UAQ8
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Synonyms

Synonyms Comment Organism
CYP719A5
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Eschscholzia californica