KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
(S)-scoulerine | - |
Eschscholzia californica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Eschscholzia californica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | Eschscholzia californica | enzyme is induced 20 h after challenging the cell suspension cultur with elicitor | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eschscholzia californica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Eschscholzia californica | - |
Storage Stability | Organism |
---|---|
-20°C, 15% loss of activity after 4 months | Eschscholzia californica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | NADPH is essential for activity, NADH displays only 0.5% turnover of that of NADPH | Eschscholzia californica | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | enzyme is induced 20 h after challenging the cell suspension cultur with elicitor | Eschscholzia californica | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Eschscholzia californica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 30 h | Eschscholzia californica |
25 | - |
half-life: 2.5 h | Eschscholzia californica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Eschscholzia californica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 50% of maximal activity at pH 7 and pH 9 | Eschscholzia californica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | the enzyme is a cytochrome P450 dependent monooxygenase | Eschscholzia californica | |
FAD | 0.004 mM, together with the optimal concentration of NADPH, 0.2 mM, enhances activity by 50% | Eschscholzia californica | |
FMN | 0.004 mM, together with the optimal concentration of NADPH, 0.2 mM, enhances activity by 50% | Eschscholzia californica |