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Literature summary for 1.14.19.64 extracted from
- Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana (2011), Arch. Biochem. Biophys., 507, 186-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Spodoptera frugiperda Sf9 cells | Argemone mexicana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.2 | - |
(S)-cheilanthifoline | pH 8.0, 30°C, kcat: 100% | Argemone mexicana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57450 | - |
calculated from cDNA | Argemone mexicana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Argemone mexicana | B1NF19 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Argemone mexicana | - |
| plantlet | - |
Argemone mexicana | - |
| root | highly expressed | Argemone mexicana | - |
| stem | - |
Argemone mexicana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| (S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2 | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP719A13 | - |
Argemone mexicana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Argemone mexicana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Argemone mexicana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at, optimal pH using S-cheilanthifoline as a substrate | Argemone mexicana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate | Argemone mexicana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana | Argemone mexicana |
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Release 2023.1 (January 2023)
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