Cloned (Comment) | Organism |
---|---|
- |
Kutzneria sp. 744 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.114 | - |
7-chloro-L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 | |
0.808 | - |
L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ | Kutzneria sp. 744 | the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) | 6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kutzneria sp. 744 | A8CF74 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Kutzneria sp. 744 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Kutzneria sp. 744 | 6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ | the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) | Kutzneria sp. 744 | 6,7-dichloro-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + Cl- + O2 + H+ | kcat/Km for the halogenation of L-tryptophan is less than 1% compared to the value for the halogenation 7-chloro-L-tryptophan | Kutzneria sp. 744 | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
additional information | KtzR is not active for introduction of chloride to piperazic acid or gamma,delta-dehydropiperazic acid | Kutzneria sp. 744 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KtzR | - |
Kutzneria sp. 744 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Kutzneria sp. 744 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 | |
0.023 | - |
7-chloro-L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Kutzneria sp. 744 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides) | Kutzneria sp. 744 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 | |
0.2 | - |
7-chloro-L-tryptophan | pH 7.0, 22°C | Kutzneria sp. 744 |