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Literature summary for 1.14.19.59 extracted from

  • Seibold, C.; Schnerr, H.; Rumpf, J.; Kunzendorf, A.; Hatscher, C.; Wage, T.; Ernyei, A.; Dong, C.; Naismith, J.; van Pee, K.
    A flavin-dependent tryptophan 6-halogenase and its use in modification of pyrrolnitrin biosynthesis (2006), Biocatal. Biotransform., 24, 401-408 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pseudomonas fluorescens and Pseudomonas chlororaphis Streptomyces albogriseolus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
L-tryptophan pH 7.2, 30°C Streptomyces albogriseolus
0.31
-
D-tryptophan pH 7.2, 30°C Streptomyces albogriseolus
1.32
-
bromide pH 7.2, 30°C Streptomyces albogriseolus
2.98
-
chloride pH 7.2, 30°C Streptomyces albogriseolus

Organism

Organism UniProt Comment Textmining
Streptomyces albogriseolus A1E280
-
-
Streptomyces albogriseolus MJ286-76F7 A1E280
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tryptophan + FADH2 + chloride + O2 + H+
-
Streptomyces albogriseolus 6-chloro-D-tryptophan + FAD + 2 H2O
-
?
D-tryptophan + FADH2 + chloride + O2 + H+
-
Streptomyces albogriseolus MJ286-76F7 6-chloro-D-tryptophan + FAD + 2 H2O
-
?
L-tryptophan + FADH2 + bromide + O2 + H+
-
Streptomyces albogriseolus 6-bromo-L-tryptophan + FAD + 2 H2O
-
?
L-tryptophan + FADH2 + bromide + O2 + H+
-
Streptomyces albogriseolus MJ286-76F7 6-bromo-L-tryptophan + FAD + 2 H2O
-
?
L-tryptophan + FADH2 + chloride + O2 + H+
-
Streptomyces albogriseolus 6-chloro-L-tryptophan + FAD + 2 H2O
-
?
L-tryptophan + FADH2 + chloride + O2 + H+
-
Streptomyces albogriseolus MJ286-76F7 6-chloro-L-tryptophan + FAD + 2 H2O
-
?
additional information enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions Streptomyces albogriseolus ?
-
?
additional information enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions Streptomyces albogriseolus MJ286-76F7 ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Streptomyces albogriseolus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
L-tryptophan pH 7.2, 30°C Streptomyces albogriseolus

General Information

General Information Comment Organism
physiological function transformation of the pyrrolnitrin producer Pseudomonas chlororaphis ACN with a plasmid containing the tryptophan 6-halogenase gene leads to the formation of the aminopyrrolnitrin derivative 3-(2'-amino-4'-chlorophenyl)pyrrole Streptomyces albogriseolus