Cloned (Comment) | Organism |
---|---|
expression in Pseudomonas fluorescens and Pseudomonas chlororaphis | Streptomyces albogriseolus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus | |
0.31 | - |
D-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus | |
1.32 | - |
bromide | pH 7.2, 30°C | Streptomyces albogriseolus | |
2.98 | - |
chloride | pH 7.2, 30°C | Streptomyces albogriseolus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces albogriseolus | A1E280 | - |
- |
Streptomyces albogriseolus MJ286-76F7 | A1E280 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus | 6-chloro-D-tryptophan + FAD + 2 H2O | - |
? | |
D-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-chloro-D-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + bromide + O2 + H+ | - |
Streptomyces albogriseolus | 6-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + bromide + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
additional information | enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions | Streptomyces albogriseolus | ? | - |
? | |
additional information | enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions | Streptomyces albogriseolus MJ286-76F7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Streptomyces albogriseolus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus |
General Information | Comment | Organism |
---|---|---|
physiological function | transformation of the pyrrolnitrin producer Pseudomonas chlororaphis ACN with a plasmid containing the tryptophan 6-halogenase gene leads to the formation of the aminopyrrolnitrin derivative 3-(2'-amino-4'-chlorophenyl)pyrrole | Streptomyces albogriseolus |