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Literature summary for 1.14.19.59 extracted from
- Characterization of a tryptophan 6-halogenase from Streptomyces toxytricini (2011), Biotechnol. Lett., 33, 1607-1613.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His6-tagged enzyme is expressed in Escherichia coli RIL | Streptomyces toxytricini |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
L-tryptophan | pH 6.0, 28°C | Streptomyces toxytricini |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, SDS-PAGE | Streptomyces toxytricini |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces toxytricini | E9P162 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces toxytricini |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-tryptophan + FADH2 + chloride + O2 + H+ = 6-chloro-D-tryptophan + FAD + 2 H2O | (1) | Streptomyces toxytricini | |
| L-tryptophan + FADH2 + chloride + O2 + H+ = 6-chloro-L-tryptophan + FAD + 2 H2O | (1) | Streptomyces toxytricini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces toxytricini | 6-chloro-D-tryptophan + FAD + 2 H2O | - |
? | |
| L-tryptophan + FADH2 + Br- + O2 + H+ | - |
Streptomyces toxytricini | 6-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
| L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces toxytricini | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, SDS-PAGE | Streptomyces toxytricini |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sttH | - |
Streptomyces toxytricini |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces toxytricini |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | 35°C: about 75% of maximal activity, 45°C: about 75% of maximal activity | Streptomyces toxytricini |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0255 | - |
L-tryptophan | pH 6.0, 28°C | Streptomyces toxytricini | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Streptomyces toxytricini |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | pH 5.0: about 75% of maximal activity, pH 7.0: about 70% of maximal activity | Streptomyces toxytricini |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-tryptophan | pH 6.0, 28°C | Streptomyces toxytricini | |
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