Cloned (Comment) | Organism |
---|---|
His-tagged apoPyrH was expressed in Pseudomonas fluorescens BL915 | Streptomyces rugosporus |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor-diffusion method at 20°C, crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD | Streptomyces rugosporus |
Protein Variants | Comment | Organism |
---|---|---|
E46D | mutant enzyme folds properly | Streptomyces rugosporus |
E46Q | mutant enzyme folds properly | Streptomyces rugosporus |
F49A/Y454F | mutant enzyme is incorrectly folded | Streptomyces rugosporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tryptophan | substrate inhibition above 0.06 mM | Streptomyces rugosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rugosporus | A4D0H5 | - |
- |
Streptomyces rugosporus LL-42D005 | A4D0H5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces rugosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces rugosporus | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces rugosporus LL-42D005 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyrH | - |
Streptomyces rugosporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces rugosporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Streptomyces rugosporus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-tryptophan | mutant enzyme E46D, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C | Streptomyces rugosporus | |
0.027 | - |
L-tryptophan | mutant enzyme F49A, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C | Streptomyces rugosporus | |
0.039 | - |
L-tryptophan | mutant enzyme E46Q, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C | Streptomyces rugosporus | |
0.05 | - |
L-tryptophan | mutant enzyme Y454F, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C | Streptomyces rugosporus | |
0.54 | - |
L-tryptophan | wild type enzyme, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C | Streptomyces rugosporus |