Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.5 extracted from

  • Ding, B.J.; Carraher, C.; Loefstedt, C.
    Sequence variation determining stereochemistry of a DELTA11 desaturase active in moth sex pheromone biosynthesis (2016), Insect Biochem. Mol. Biol., 74, 68-75 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Saccharomyces cerevisiae double deficient ole1 elo1 strain Choristoneura rosaceana

Protein Variants

Protein Variants Comment Organism
16del_E site-directed mutagenesis, the product E/Z isomer ratio is 33:67 Choristoneura rosaceana
A161G/T163M site-directed mutagenesis, the product E/Z isomer ratio is 36:64 Choristoneura rosaceana
A88S site-directed mutagenesis, the product E/Z isomer ratio is 32:68 Choristoneura rosaceana
E258D site-directed mutagenesis, mutating the glutamic acid into aspartic acid transforms the Choristoneura rosaceana enzyme into a desaturase with Choristoneura parallela-like activity that produces an almost pure (E)-11-tetradecenoate product, the product E/Z isomer ratio is 86:14 Choristoneura rosaceana
F252L site-directed mutagenesis, the product E/Z isomer ratio is 33:67 Choristoneura rosaceana
H116N/I118V site-directed mutagenesis, the product E/Z isomer ratio is 42:58 Choristoneura rosaceana
I103M site-directed mutagenesis, the product E/Z isomer ratio is 36:64 Choristoneura rosaceana
I174V site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
I65V site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
K286Q site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
K309N site-directed mutagenesis, the product E/Z isomer ratio is 34:66 Choristoneura rosaceana
L12M site-directed mutagenesis, the product E/Z isomer ratio is 31:69 Choristoneura rosaceana
L19W site-directed mutagenesis, the product E/Z isomer ratio is 34:66 Choristoneura rosaceana
L69I site-directed mutagenesis, the product E/Z isomer ratio is 30:70 Choristoneura rosaceana
M250I/T251A site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
N259S site-directed mutagenesis, the product E/Z isomer ratio is 31:69 Choristoneura rosaceana
Q33E site-directed mutagenesis, inactive mutant Choristoneura rosaceana
S109A site-directed mutagenesis, inactive mutant Choristoneura rosaceana
T95A site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
V321I site-directed mutagenesis, the product E/Z isomer ratio is 35:65 Choristoneura rosaceana
W45G site-directed mutagenesis, the product E/Z isomer ratio is 31:69 Choristoneura rosaceana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane topological model of CroD11 desaturase spanning the endoplasmic reticulum membrane Choristoneura rosaceana 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myristic acid + 2 ferrocytochrome b5 + O2 + 2 H+ Choristoneura rosaceana
-
(Z)-11-tetradecenoate + (E)-11-tetradecenoate + 2 ferricytochrome b5 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Choristoneura rosaceana Q8ISS3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information structure-function analysis determing the stereochemistry of the product formation. Residue E258 in the cytosolic carboxyl terminus of the protein is critical for the Z activity of the Choristoneura rosaceana desaturase Choristoneura rosaceana ?
-
?
myristic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Choristoneura rosaceana (Z)-11-tetradecenoate + (E)-11-tetradecenoate + 2 ferricytochrome b5 + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
acyl-CoA Z/E11 desaturase UniProt Choristoneura rosaceana
CroDELTA11 desaturase
-
Choristoneura rosaceana
DELTA11 desaturase
-
Choristoneura rosaceana
More cf. EC 1.14.19.24 Choristoneura rosaceana

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Choristoneura rosaceana

General Information

General Information Comment Organism
evolution the enzyme belongs to the fatty acid desaturase type 1 family Choristoneura rosaceana
additional information the 258E/D residue contributes to the formation of the secondary coordination sphere of the dimetal unit, along with 129D, 133D and 228N, molecular docking study, overview. This residue might be influencing the shape of the reactive cavity and may play an important role in the catalytic property of this desaturase. Residue E258 in the cytosolic carboxyl terminus of the protein is critical for the steroechmistry of activity Choristoneura rosaceana