BRENDA - Enzyme Database show
show all sequences of 1.14.19.5

Characterization of the DELTA11-palmitoyl-CoA-desaturase from Spodoptera littoralis (Lepidoptera:Noctuidae)

Rodriguez, F.; Hallahan, D.L.; Pickett, J.A.; Camps, F.; Insect Biochem. Mol. Biol. 22, 143-148 (1992)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
83% inhibition by 1 mM, 95% inhibition by 5 mM
Spodoptera littoralis
NaN3
92% inhibition b
Spodoptera littoralis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Spodoptera littoralis
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spodoptera littoralis
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
pheromone gland
-
Spodoptera littoralis
-
Storage Stability
Storage Stability
Organism
-80C, stable for at least 1 h
Spodoptera littoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
myristic acid + NADH + ?
50% of the activity with palmitic acid
658905
Spodoptera littoralis
tetradec-11-enoic acid + NAD+ + ?
-
-
-
?
palmitic acid + NADH + ?
-
658905
Spodoptera littoralis
(Z)-hexadec-11-enoic acid + NAD+ + ?
-
-
-
?
palmitoyl-CoA + NADH + ?
-
658905
Spodoptera littoralis
(Z)-hexadec-11-enoyl-CoA + NAD+ + ?
-
-
-
?
stearic acid + NADH + ?
18% of the activity with palmitic acid
658905
Spodoptera littoralis
oleic acid + NAD+ + ?
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Spodoptera littoralis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
-
Spodoptera littoralis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is a less effective elctron donor. Highest activity with 1 mM of electron donors, reduced activity below
Spodoptera littoralis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is a less effective elctron donor. Highest activity with 1 mM of electron donors, reduced activity below
Spodoptera littoralis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
83% inhibition by 1 mM, 95% inhibition by 5 mM
Spodoptera littoralis
NaN3
92% inhibition b
Spodoptera littoralis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Spodoptera littoralis
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
pheromone gland
-
Spodoptera littoralis
-
Storage Stability (protein specific)
Storage Stability
Organism
-80C, stable for at least 1 h
Spodoptera littoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
myristic acid + NADH + ?
50% of the activity with palmitic acid
658905
Spodoptera littoralis
tetradec-11-enoic acid + NAD+ + ?
-
-
-
?
palmitic acid + NADH + ?
-
658905
Spodoptera littoralis
(Z)-hexadec-11-enoic acid + NAD+ + ?
-
-
-
?
palmitoyl-CoA + NADH + ?
-
658905
Spodoptera littoralis
(Z)-hexadec-11-enoyl-CoA + NAD+ + ?
-
-
-
?
stearic acid + NADH + ?
18% of the activity with palmitic acid
658905
Spodoptera littoralis
oleic acid + NAD+ + ?
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Spodoptera littoralis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
-
Spodoptera littoralis
Other publictions for EC 1.14.19.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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221
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1
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7
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1
1
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745075
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Multiple DELTA11-desaturase g ...
Ostrinia nubilalis, Ostrinia scapulalis
Insect Biochem. Mol. Biol.
61
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2015
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726885
Fujii
Discovery of a disused desatur ...
Ascotis selenaria
Biochem. Biophys. Res. Commun.
441
849-855
2013
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713433
Fujii
Sex pheromone desaturase funct ...
Ostrinia latipennis
Proc. Natl. Acad. Sci. USA
108
7102-7106
2011
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1
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712163
Lienard
Elucidation of the sex-pheromo ...
Dendrolimus punctatus
Insect Biochem. Mol. Biol.
40
440-452
2010
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1
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1
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698285
Sakai
Alternative suppression of tra ...
Ostrinia furnacalis, Ostrinia scapulalis
Insect Biochem. Mol. Biol.
39
62-67
2009
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1
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2
2
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696969
Lienard
Key biosynthetic gene subfamil ...
Lampronia capitella
BMC Evol. Biol.
8
270
2008
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1
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1
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5
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1
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687090
Matouskova
Functional characterization of ...
Manduca sexta
Insect Biochem. Mol. Biol.
37
601-610
2007
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1
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674009
Serra
Biosynthesis of 10,12-dienoic ...
Spodoptera littoralis
Insect Biochem. Mol. Biol.
36
634-641
2006
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1
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674010
Serra
DELTA11 desaturases of Trichop ...
Spodoptera littoralis, Trichoplusia ni
Insect Biochem. Mol. Biol.
36
822-825
2006
-
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2
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675253
Fukuzawa
cDNA cloning and in situ hybri ...
Ostrinia scapulalis
J. Insect Physiol.
52
430-435
2006
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658737
Tonon
Identification of a fatty acid ...
Thalassiosira pseudonana
FEBS Lett.
563
28-34
2004
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658912
Rodriguez
Expression and evolution of DE ...
Spodoptera littoralis
Insect Biochem. Mol. Biol.
34
1315-1328
2004
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659777
Abad
Synthesis and use of stereospe ...
Thaumetopoea pityocampa
J. Org. Chem.
69
7108-7113
2004
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659834
Abad
Synthesis of fluorinated analo ...
Spodoptera littoralis
Lipids
38
865-871
2003
-
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1
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688572
Villorbina
Enzymatic desaturation of fatt ...
Spodoptera littoralis
J. Org. Chem.
68
2820-2829
2003
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658910
Liu
Moth desaturase characterized ...
Argyrotaenia velutinana
Insect Biochem. Mol. Biol.
32
1489-1495
2002
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659648
Hao
Characterization of Z/E11- and ...
Choristoneura rosaceana
J. Insect Sci.
2
26
2002
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1
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4
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285465
Pinilla
Cryptoregiochemistry of the de ...
Spodoptera littoralis
Biochemistry
38
15272-15277
1999
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658907
Svatos
-
Stereochemistry of lepidoptera ...
Bombyx mori, Manduca sexta
Insect Biochem. Mol. Biol.
29
225-232
1999
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660398
Knipple
Cloning and functional express ...
Trichoplusia ni
Proc. Natl. Acad. Sci. USA
95
15287-15292
1998
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-
-
-
-
-
-
-
-
-
-
-
-
285464
Navarro
-
Stereospecificity of the (E)- ...
Spodoptera littoralis
J. Am. Chem. Soc.
119
11335-11336
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
658905
Rodriguez
-
Characterization of the DELTA1 ...
Spodoptera littoralis
Insect Biochem. Mol. Biol.
22
143-148
1992
-
-
-
-
-
-
2
-
1
-
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1
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4
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1
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-
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-
-
659562
Wolf
Reinvestigation confirms actio ...
Choristoneura fumiferana
J. Chem. Ecol.
13
1019-1027
1987
-
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1
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