Literature summary for 1.14.19.45 extracted from

Gerasymenko, I.; Sakhno, L.; Kyrpa, T.; Ostapchuk, A.; Hadjiev, T.; Goldenkova-Pavlova, I.; Sheludko, Y.
Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial DELTA9 or DELTA12 acyl-lipid desaturases and thermostable lichenase (2015), Russ. J. Plant Physiol., 62, 283-291 .

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of hybrid gene of Synechococcus vulcanus DELTA12 (desA) acyllipid desaturase in Nicotiana tabacum cv. Wisconsin, as chimeric enzyme DesA-LicBM3 with reporter thermostable lichenase of Clostridium thermocellum, expression with or without the sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of the 35S CaMV constitutive promoter using the Agrobacterium tumefaciens strain GV3101-mediated transformation method, measurement of recombinant lichenase activity. Plants expressing RTP::desA::licBM3 gene coding for DELTA12 desaturase with the transit peptide for transport into chloroplasts causes a reliable increase of DELTA9,12-18:2 fatty acids and a decrease of DELTA9,12,15-18:3 fatty acids	Synechocystis sp.
transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter are established	Synechocystis sp. PCC 6803

Cloned (Comment)

Organism

recombinant expression of hybrid gene of Synechococcus vulcanus DELTA12 (desA) acyllipid desaturase in Nicotiana tabacum cv. Wisconsin, as chimeric enzyme DesA-LicBM3 with reporter thermostable lichenase of Clostridium thermocellum, expression with or without the sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of the 35S CaMV constitutive promoter using the Agrobacterium tumefaciens strain GV3101-mediated transformation method, measurement of recombinant lichenase activity. Plants expressing RTP::desA::licBM3 gene coding for DELTA12 desaturase with the transit peptide for transport into chloroplasts causes a reliable increase of DELTA9,12-18:2 fatty acids and a decrease of DELTA9,12,15-18:3 fatty acids

Synechocystis sp.

transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter are established

Synechocystis sp. PCC 6803

Protein Variants

Protein Variants	Comment	Organism
additional information	establishment of transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2, DELTA9,12) accompanied with decrease of alpha-linolenic acid (C18:3, DELTA9,12,15) relative amount is detected for plants expressing hybrid desA::licBM3 gene. No reliable changes are detected in fatty acid profiles and unsaturation index of plants transformed with DELTA9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 alpha-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsaturation index. No changes in relative amount of DELTA9monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment	Synechocystis sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	membrane-bound	Synechocystis sp.	16020	-

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp.	P20388	-	-
Synechocystis sp. PCC 6803	P20388	-	-

Synonyms

Synonyms	Comment	Organism
acyl-lipid DELTA12 desaturase	-	Synechocystis sp.
DELTA12 acyl-lipid desaturase	-	Synechocystis sp.
DELTA12-acyl-lipid desaturase	-	Synechocystis sp. PCC 6803
DesA	-	Synechocystis sp.
DesA	-	Synechocystis sp. PCC 6803