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Literature summary for 1.14.19.45 extracted from
- DELTA12-Fatty acid desaturase from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids (2014), PLoS ONE, 9, e93322 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis | Candida parapsilosis |
| overexpression in Saccharomyces cerevisiae | Candida parapsilosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Candida parapsilosis | analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview | ? | - |
? |  |
| additional information | Candida parapsilosis CP-69 | analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida parapsilosis | C3W956 | - |
- |
| Candida parapsilosis CP-69 | C3W956 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview | Candida parapsilosis | ? | - |
? | |
| additional information | the enzyme produces a range of DELTA12- and DELTA15-polyunsaturated fatty acids. The major products of CpFad2 are linoleic and hexadecadienoic acid (D9,D12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, ricinoleic acid (12-hydroxy-9-octadecenoic acid) is identified as an additional product | Candida parapsilosis | ? | - |
? | |
| additional information | unambiguous identification of the triunsaturated product of CpFad2 as DELTA9,DELTA12,DELTA15-16:3 with terminal (n-1) double bond by comparing its spectrum to that of DELTA9,DELTA12,DELTA15-16:3 methylester | Candida parapsilosis | ? | - |
? | |
| additional information | analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview | Candida parapsilosis CP-69 | ? | - |
? | |
| additional information | the enzyme produces a range of DELTA12- and DELTA15-polyunsaturated fatty acids. The major products of CpFad2 are linoleic and hexadecadienoic acid (D9,D12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, ricinoleic acid (12-hydroxy-9-octadecenoic acid) is identified as an additional product | Candida parapsilosis CP-69 | ? | - |
? | |
| additional information | unambiguous identification of the triunsaturated product of CpFad2 as DELTA9,DELTA12,DELTA15-16:3 with terminal (n-1) double bond by comparing its spectrum to that of DELTA9,DELTA12,DELTA15-16:3 methylester | Candida parapsilosis CP-69 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpFAD2 | - |
Candida parapsilosis |
| CpFAD2 | DELTA12/DELTA15-fatty acid desaturase | Candida parapsilosis |
| DELTA12-fatty acid desaturase | - |
Candida parapsilosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | DELTA12-fatty acid desaturase CpFAD2 from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids | Candida parapsilosis |
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