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Literature summary for 1.14.19.45 extracted from

  • Cui, J.; He, S.; Ji, X.; Lin, L.; Wei, Y.; Zhang, Q.
    Identification and characterization of a novel bifunctional DELTA12/DELTA15-fatty acid desaturase gene from Rhodosporidium kratochvilovae (2016), Biotechnol. Lett., 38, 1155-1164 .
    View publication on PubMed
Search for more literature for 1.14.19.45

Cloned(Commentary)

Cloned (Comment) Organism
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae Rhodotorula kratochvilovae
RKD12 is further transformed into Saccharomyces cerevisiae for functional characterization Rhodotorula kratochvilovae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 1-oleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Rhodotorula kratochvilovae
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 a 1-linoleoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
additional information Rhodotorula kratochvilovae the enzyme produces linoleic acid and alpha-linolenic acid, overview ?
-
 ?
additional information Rhodotorula kratochvilovae YM25235 the enzyme produces linoleic acid and alpha-linolenic acid, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodotorula kratochvilovae A0A024B710
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-
Rhodotorula kratochvilovae A0A024B710 isolated from Chenghai Lake in Yunnan, China
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Rhodotorula kratochvilovae YM25235 A0A024B710
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Rhodotorula kratochvilovae YM25235 A0A024B710 isolated from Chenghai Lake in Yunnan, China
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 1-oleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Rhodotorula kratochvilovae a 1-linoleoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
additional information the enzyme produces linoleic acid and alpha-linolenic acid, overview Rhodotorula kratochvilovae ?
-
 ?
additional information absence of desaturation activity of RKD12 for C16 substrates Rhodotorula kratochvilovae ?
-
 ?
additional information the enzyme produces linoleic acid and alpha-linolenic acid, overview Rhodotorula kratochvilovae YM25235 ?
-
 ?
additional information absence of desaturation activity of RKD12 for C16 substrates Rhodotorula kratochvilovae YM25235 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50600, about, sequence calculation Rhodotorula kratochvilovae
? x * 50634, calculated from sequence Rhodotorula kratochvilovae

Synonyms

Synonyms Comment Organism
bifunctional DELTA12/DELTA15-fatty acid desaturase
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 Rhodotorula kratochvilovae
DELTA(12) fatty acid desaturase
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 Rhodotorula kratochvilovae
DELTA12-desaturase
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 Rhodotorula kratochvilovae
More cf. EC 1.14.19.13 Rhodotorula kratochvilovae
RKD12
-
 Rhodotorula kratochvilovae
RKD12 bifunctional DELTA12/DELTA15-desaturase gene Rhodotorula kratochvilovae

Expression

Organism Comment Expression
Rhodotorula kratochvilovae a culture temperature downshift from 28°C to 15°C induces the enzyme up

General Information

General Information Comment Organism
evolution bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference Rhodotorula kratochvilovae
physiological function for better adaptation to low temperatures, YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving th enzyme Rhodotorula kratochvilovae
physiological function increased RKD12 mRNA expression level and PUFAs content at low temperature might be helpful for the cold adaptation of Rhodosporidium kratochvilovae YM25235 Rhodotorula kratochvilovae