Cloned (Comment) | Organism |
---|---|
expressed in a baculovirus/Sf9 insect cell system | Physcomitrium patens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
beta-sitosterol | isoform CAP710A13, at pH 7.3 and 30°C | Physcomitrium patens | |
0.0021 | - |
beta-sitosterol | isoform CAP710A14, at pH 7.3 and 30°C | Physcomitrium patens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Physcomitrium patens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57028 | - |
x * 57028, isoform CYP710A14, calculated from amino acid sequence | Physcomitrium patens |
57410 | - |
x * 57410, isoform CYP710A13, calculated from amino acid sequence | Physcomitrium patens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + NADPH + H+ + O2 | Physcomitrium patens | - |
stigmasterol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physcomitrium patens | - |
subspecies Physcomitrella patens patens, strain Gransden Wood | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + NADPH + H+ + O2 | - |
Physcomitrium patens | stigmasterol + NADP+ + H2O | - |
? | |
additional information | campesterol and 24-epi-campesterol are not accepted as substrates | Physcomitrium patens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000-57000, SDS-PAGE | Physcomitrium patens |
? | x * 57028, isoform CYP710A14, calculated from amino acid sequence | Physcomitrium patens |
? | x * 57410, isoform CYP710A13, calculated from amino acid sequence | Physcomitrium patens |
Synonyms | Comment | Organism |
---|---|---|
CYP710A | - |
Physcomitrium patens |
CYP710A13 | isoform | Physcomitrium patens |
CYP710A14 | isoform | Physcomitrium patens |
sterol C22-desaturase | - |
Physcomitrium patens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
beta-sitosterol | isoform CAP710A14, at pH 7.3 and 30°C | Physcomitrium patens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Physcomitrium patens |