BRENDA - Reference to 1.14.19.4; Id = 746465

genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis

Solanum lycopersicum

additional information

a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants

Solanum lycopersicum

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cv. Micro-Tom

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fruit

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Solanum lycopersicum

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leaf

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Solanum lycopersicum

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SlSLD

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Solanum lycopersicum

sphingolipid delta8 desaturase

-

Solanum lycopersicum

genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis

Solanum lycopersicum

additional information

a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants

Solanum lycopersicum

fruit

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Solanum lycopersicum

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leaf

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Solanum lycopersicum

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Solanum lycopersicum

gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit

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malfunction

in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking

Solanum lycopersicum

metabolism

SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism

Solanum lycopersicum

physiological function

the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism

Solanum lycopersicum

malfunction

in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking

Solanum lycopersicum

metabolism

SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism

Solanum lycopersicum

physiological function

the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism

Solanum lycopersicum

gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit

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746465

Zhou

The sphingolipid biosynthetic ...

Solanum lycopersicum

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746153

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Candida albicans

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673544

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658684

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657894

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Aquilegia vulgaris

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Curr. Microbiol.

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684433

Beckmann Christop

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657640

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Functional identification of a ...

Borago officinalis

Arch. Biochem. Biophys.

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279

779-785

2000

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657892

Sperling

Further characterization of DE ...

Helianthus annuus

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638-641

2000

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659831

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The questionable role of a mic ...

Rattus norvegicus

Lipids

35

871-879

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657637

Wallis

The DELTA8-desaturase of Eugle ...

Euglena gracilis

Arch. Biochem. Biophys.

365

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1

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6

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1

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3

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1

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1

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3

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The sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato

Data extracted from this reference: