BRENDA - Enzyme Database
show all sequences of 1.14.19.4

The sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato

Zhou, Y.; Zeng, L.; Fu, X.; Mei, X.; Cheng, S.; Liao, Y.; Deng, R.; Xu, X.; Jiang, Y.; Duan, X.; Baldermann, S.; Yang, Z.; Sci. Rep. 6, 38742 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis
Solanum lycopersicum
Engineering
Protein Variants
Commentary
Organism
additional information
a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants
Solanum lycopersicum
Organism
Organism
UniProt
Commentary
Textmining
Solanum lycopersicum
-
cv. Micro-Tom
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-
Synonyms
Synonyms
Commentary
Organism
SlSLD
-
Solanum lycopersicum
sphingolipid delta8 desaturase
-
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis
Solanum lycopersicum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants
Solanum lycopersicum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Solanum lycopersicum
-
leaf
-
Solanum lycopersicum
-
Expression
Organism
Commentary
Expression
Solanum lycopersicum
gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit
up
General Information
General Information
Commentary
Organism
malfunction
in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking
Solanum lycopersicum
metabolism
SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism
Solanum lycopersicum
physiological function
the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking
Solanum lycopersicum
metabolism
SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism
Solanum lycopersicum
physiological function
the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism
Solanum lycopersicum
Expression (protein specific)
Organism
Commentary
Expression
Solanum lycopersicum
gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit
up
Other publictions for EC 1.14.19.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746465
Zhou
The sphingolipid biosynthetic ...
Solanum lycopersicum
Sci. Rep.
6
38742
2016
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Nagano
Arabidopsis Bax inhibitor-1 p ...
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Planta
240
77-89
2014
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726865
Li
Newly identified essential ami ...
Brassica rapa
Biochem. Biophys. Res. Commun.
416
165-171
2011
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Habel
Conformational studies on the ...
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J. Org. Chem.
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An alternate pathway to long-c ...
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Candida albicans
Microbiology
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689583
Ryan
A higher plant DELTA8 sphingol ...
Arabidopsis thaliana, Stylosanthes hamata
Plant Physiol.
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2007
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Tripodi
Functional characterization of ...
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The alternative pathway C20 DE ...
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658684
Tonon
Fatty acid desaturases from th ...
Thalassiosira pseudonana
FEBS J.
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3401-3412
2005
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657894
Michaelson
Isolation and characterization ...
Aquilegia vulgaris
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Takakuwa
Isolation and characterization ...
Kluyveromyces lactis, Lachancea kluyveri
Curr. Microbiol.
45
459-461
2002
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684433
Beckmann Christop
Characterization of a DELTA8-s ...
Helianthus annuus, no activity in Saccharomyces cerevisiae
Angew. Chem. Int. Ed. Engl.
41
2298-2300
2002
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657640
Sperling
Functional identification of a ...
Borago officinalis
Arch. Biochem. Biophys.
388
293-298
2001
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657719
Libisch
Chimeras of DELTA6-fatty acid ...
Borago officinalis
Biochem. Biophys. Res. Commun.
279
779-785
2000
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657892
Sperling
Further characterization of DE ...
Helianthus annuus
Biochem. Soc. Trans.
28
638-641
2000
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659831
Chen
The questionable role of a mic ...
Rattus norvegicus
Lipids
35
871-879
2000
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657637
Wallis
The DELTA8-desaturase of Eugle ...
Euglena gracilis
Arch. Biochem. Biophys.
365
307-316
1999
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