Data extracted from this reference:
Cloned(Commentary)
genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis
Solanum lycopersicum
Engineering
additional information
a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants
Solanum lycopersicum
Organism
Solanum lycopersicum
cv. Micro-Tom
Source Tissue
fruit
Solanum lycopersicum
leaf
Solanum lycopersicum
Synonyms
SlSLD
Solanum lycopersicum
sphingolipid delta8 desaturase
Solanum lycopersicum
Cloned(Commentary) (protein specific)
genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis
Solanum lycopersicum
Engineering (protein specific)
additional information
a virus-induced gene silencing (VIGS) approach to knockdown gene SlSLD expression is used in tomato leaves involving transformation via Agrobacterium GV3101, chilling resistance is evaluated for wild-type and mutangt plant seedlings, determination of the mutant phenotype, detailed overview. After exposure to 4°C, SlSLD-silenced mutant tomato plants are severely damaged. Exposure to low temperature severely damages chloroplasts in SlSLD-silenced plants
Solanum lycopersicum
Source Tissue (protein specific)
fruit
Solanum lycopersicum
leaf
Solanum lycopersicum
Expression
Solanum lycopersicum
gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit
up
General Information
malfunction
in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking
Solanum lycopersicum
metabolism
SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism
Solanum lycopersicum
physiological function
the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism
Solanum lycopersicum
General Information (protein specific)
malfunction
in enzyme knockout plants, changes in leaf cell structure under a chilling treatment are observed by transmission electron microscopy. The degree of chilling damage is greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knockdown significantly reduces the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants show higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damages the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking
Solanum lycopersicum
metabolism
SlSLD1 and 2 are important chilling stress-responsive genes involved in sphingolipid metabolism
Solanum lycopersicum
physiological function
the sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato. The enzyme encoding gene SlSLD belongs to the chilling stress-responsive genes related to sphingolipid metabolism
Solanum lycopersicum
Expression (protein specific)
Solanum lycopersicum
gene SlSLD is highly expressed in the fruit and leaves in response to a chilling treatment. After the chilling treatment, the transcript levels of SlSLD1 are increased 23.4fold in leaves and 2.9fold in fruit, and the transcript levels of SlSLD2 are increased 8.4fold in leaves and 2.4fold in fruit
up
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