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Literature summary for 1.14.19.4 extracted from

  • Nagano, M.; Ishikawa, T.; Ogawa, Y.; Iwabuchi, M.; Nakasone, A.; Shimamoto, K.; Uchimiya, H.; Kawai-Yamada, M.
    Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes (2014), Planta, 240, 77-89 .
    View publication on PubMed
Search for more literature for 1.14.19.4

Activating Compound

Activating Compound Comment Organism Structure
Bax inhibitor-1 from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. AtBI-1 interacts with AtSLD2 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs Arabidopsis thaliana
Bax inhibitor-1 from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. The AtBI-1 protein expression is induces in cold exposure. AtBI-1 contributes to synthesis of sphingolipids during cold stress by interacting with AtSLD1 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
gene AtSLD1, sequence comparisons, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells, coexpression of AtBI-1-cYFP and AtSLD1-nYFP Arabidopsis thaliana
gene AtSLD2, sequence comparions, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of isozymes SLD1 and SLD2 knockout mutant, sphingolipid content in sld1sld2 and Bax inhibitor-1 atbi-1 mutant during cold treatment, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Arabidopsis thaliana 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview ?
-
 ?
additional information Arabidopsis thaliana Col-0 desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q3EBF7
-
-
Arabidopsis thaliana Q9ZRP7
-
-
Arabidopsis thaliana Col-0 Q3EBF7
-
-
Arabidopsis thaliana Col-0 Q9ZRP7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
additional information expression of isozyme AtSLD2 is limited to flowers and siliques. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed Arabidopsis thaliana
-
additional information isozyme AtSLD1 is broadly expressed in all tissues. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview Arabidopsis thaliana ?
-
 ?
additional information desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

Synonyms Comment Organism
AtSLD1
-
 Arabidopsis thaliana
AtSLD2
-
 Arabidopsis thaliana
sphingolipid DELTA8 LCB desaturase
-
 Arabidopsis thaliana
sphingolipid DELTA8 long-chain base desaturase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 a Cb5-like domain (Cb5LD)-containing protein Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the expression of AtSLD2 is decreased at cold exposure down
Arabidopsis thaliana the expression of AtSLD1 is increased at cold exposure up

General Information

General Information Comment Organism
physiological function isozymes AtSLD1 and AtSLD2 desaturate the DELTA8 position of the long-chain bases (LCBs) of sphingolipids in the endoplasmic reticulum membrane, and include a cytochrome b5-like domain at the N-terminus. 90% of Arabidopsis thaliana long-chain bases contain a cis or trans double bond at the DELTA8 position, and their desaturation is catalyzed by AtSLD1 and AtSLD2 Arabidopsis thaliana