BRENDA - Enzyme Database
show all sequences of 1.14.19.4

Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes

Nagano, M.; Ishikawa, T.; Ogawa, Y.; Iwabuchi, M.; Nakasone, A.; Shimamoto, K.; Uchimiya, H.; Kawai-Yamada, M.; Planta 240, 77-89 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Bax inhibitor-1
from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. AtBI-1 interacts with AtSLD2 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs; from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. The AtBI-1 protein expression is induces in cold exposure. AtBI-1 contributes to synthesis of sphingolipids during cold stress by interacting with AtSLD1 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs
Arabidopsis thaliana
Cloned(Commentary)
Cloned (Commentary)
Organism
gene AtSLD1, sequence comparisons, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells, coexpression of AtBI-1-cYFP and AtSLD1-nYFP; gene AtSLD2, sequence comparions, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
additional information
generation of isozymes SLD1 and SLD2 knockout mutant, sphingolipid content in sld1sld2 and Bax inhibitor-1 atbi-1 mutant during cold treatment, overview; generation of isozymes SLD1 and SLD2 knockout mutant, sphingolipid content in sld1sld2 and Bax inhibitor-1 atbi-1 mutant during cold treatment, overview
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum membrane
;
Arabidopsis thaliana
5789
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Arabidopsis thaliana
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
?
-
-
-
additional information
Arabidopsis thaliana Col-0
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q3EBF7
-
-
Arabidopsis thaliana
Q9ZRP7
-
-
Arabidopsis thaliana Col-0
Q3EBF7
-
-
Arabidopsis thaliana Col-0
Q9ZRP7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
;
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
expression of isozyme AtSLD2 is limited to flowers and siliques. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed; isozyme AtSLD1 is broadly expressed in all tissues. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
;
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
746153
Arabidopsis thaliana
?
-
-
-
-
additional information
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
746153
Arabidopsis thaliana Col-0
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
AtSLD1
-
Arabidopsis thaliana
AtSLD2
-
Arabidopsis thaliana
sphingolipid DELTA8 LCB desaturase
-
Arabidopsis thaliana
sphingolipid DELTA8 long-chain base desaturase
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
a Cb5-like domain (Cb5LD)-containing protein; a Cb5-like domain (Cb5LD)-containing protein
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Bax inhibitor-1
from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. The AtBI-1 protein expression is induces in cold exposure. AtBI-1 contributes to synthesis of sphingolipids during cold stress by interacting with AtSLD1 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs
Arabidopsis thaliana
Bax inhibitor-1
from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. AtBI-1 interacts with AtSLD2 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene AtSLD1, sequence comparisons, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells, coexpression of AtBI-1-cYFP and AtSLD1-nYFP
Arabidopsis thaliana
gene AtSLD2, sequence comparions, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
a Cb5-like domain (Cb5LD)-containing protein
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of isozymes SLD1 and SLD2 knockout mutant, sphingolipid content in sld1sld2 and Bax inhibitor-1 atbi-1 mutant during cold treatment, overview
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum membrane
-
Arabidopsis thaliana
5789
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Arabidopsis thaliana
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
?
-
-
-
additional information
Arabidopsis thaliana Col-0
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
isozyme AtSLD1 is broadly expressed in all tissues. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed
Arabidopsis thaliana
-
additional information
expression of isozyme AtSLD2 is limited to flowers and siliques. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
746153
Arabidopsis thaliana
?
-
-
-
-
additional information
desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview
746153
Arabidopsis thaliana Col-0
?
-
-
-
-
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
the expression of AtSLD2 is decreased at cold exposure
down
Arabidopsis thaliana
the expression of AtSLD1 is increased at cold exposure
up
General Information
General Information
Commentary
Organism
physiological function
isozymes AtSLD1 and AtSLD2 desaturate the DELTA8 position of the long-chain bases (LCBs) of sphingolipids in the endoplasmic reticulum membrane, and include a cytochrome b5-like domain at the N-terminus. 90% of Arabidopsis thaliana long-chain bases contain a cis or trans double bond at the DELTA8 position, and their desaturation is catalyzed by AtSLD1 and AtSLD2; isozymes AtSLD1 and AtSLD2 desaturate the DELTA8 position of the long-chain bases (LCBs) of sphingolipids in the endoplasmic reticulum membrane, and include a cytochrome b5-like domain at the N-terminus. 90% of Arabidopsis thaliana long-chain bases contain a cis or trans double bond at the DELTA8 position, and their desaturation is catalyzed by AtSLD1 and AtSLD2
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
isozymes AtSLD1 and AtSLD2 desaturate the DELTA8 position of the long-chain bases (LCBs) of sphingolipids in the endoplasmic reticulum membrane, and include a cytochrome b5-like domain at the N-terminus. 90% of Arabidopsis thaliana long-chain bases contain a cis or trans double bond at the DELTA8 position, and their desaturation is catalyzed by AtSLD1 and AtSLD2
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
the expression of AtSLD2 is decreased at cold exposure
down
Arabidopsis thaliana
the expression of AtSLD1 is increased at cold exposure
up
Other publictions for EC 1.14.19.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746465
Zhou
The sphingolipid biosynthetic ...
Solanum lycopersicum
Sci. Rep.
6
38742
2016
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1
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1
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1
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746153
Nagano
Arabidopsis Bax inhibitor-1 p ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
240
77-89
2014
1
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2
1
2
2
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726865
Li
Newly identified essential ami ...
Brassica rapa
Biochem. Biophys. Res. Commun.
416
165-171
2011
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15
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1
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712836
Habel
Conformational studies on the ...
Helianthus annuus
J. Org. Chem.
75
4975-4982
2010
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1
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1
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699412
Park
An alternate pathway to long-c ...
Papio anubis
J. Lipid Res.
50
1195-1202
2009
-
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1
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1
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700049
Oura
Disruption of the sphingolipid ...
Candida albicans
Microbiology
154
3795-3803
2008
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1
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6
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2
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689583
Ryan
A higher plant DELTA8 sphingol ...
Arabidopsis thaliana, Stylosanthes hamata
Plant Physiol.
144
1968-1977
2007
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2
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3
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8
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1
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673544
Tripodi
Functional characterization of ...
Leishmania major
FEBS J.
273
271-280
2006
-
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1
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10
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673639
Sayanova
The alternative pathway C20 DE ...
Acanthamoeba castellanii
FEBS Lett.
580
1946-1952
2006
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1
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1
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6
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658684
Tonon
Fatty acid desaturases from th ...
Thalassiosira pseudonana
FEBS J.
272
3401-3412
2005
-
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3
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657894
Michaelson
Isolation and characterization ...
Aquilegia vulgaris
Biochem. Soc. Trans.
30
1073-1075
2002
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1
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4
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658490
Takakuwa
Isolation and characterization ...
Kluyveromyces lactis, Lachancea kluyveri
Curr. Microbiol.
45
459-461
2002
-
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2
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2
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684433
Beckmann Christop
Characterization of a DELTA8-s ...
Helianthus annuus, no activity in Saccharomyces cerevisiae
Angew. Chem. Int. Ed. Engl.
41
2298-2300
2002
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1
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3
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1
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1
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657640
Sperling
Functional identification of a ...
Borago officinalis
Arch. Biochem. Biophys.
388
293-298
2001
-
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1
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4
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1
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657719
Libisch
Chimeras of DELTA6-fatty acid ...
Borago officinalis
Biochem. Biophys. Res. Commun.
279
779-785
2000
-
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1
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1
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