BRENDA - Enzyme Database
show all sequences of 1.14.19.4

A higher plant DELTA8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants

Ryan, P.R.; Liu, Q.; Sperling, P.; Dong, B.; Franke, S.; Delhaize, E.; Plant Physiol. 144, 1968-1977 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae confers tolerance to aluminium to the cells
Arabidopsis thaliana
gene S581, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae and constitutively in Arabidopsis thaliana, under control of the CaMV35S promoter, confers tolerance to aluminium and gadolinium, Gd3+, but not to lanthanum, La3+, or manganese, Mn2+, to cells and seedlings, respectively, the transgenic expression leads to the accumulation of 8(Z/E)-C18-phytosphingenine and 8(Z/E)-C20-phytopshingenine in yeast and to the accumulation of 8(Z/E)-C18-phytosphingenine in the leaves and roots of Arabidopsis plants, overview
Stylosanthes hamata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DELTA4-sphingenine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA4,8-sphingadienine + acceptor + 2 H2O
production of two water molecules in the reaction
-
?
phytosphinganine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA8-phytosphingenine + acceptor + H2O
production of two water molecules in the reaction
-
?
sphinganine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA8-sphingenine + acceptor + 2 H2O
production of two water molecules in the reaction
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
AF001394
-
-
Stylosanthes hamata
A7Y7E1
gene S581
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Stylosanthes hamata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
DELTA4-sphingenine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA4,8-sphingadienine + acceptor + 2 H2O
production of two water molecules in the reaction
-
-
?
additional information
DELTA8 sphingolipid desaturase creates double bonds in long-chain bases, i.e. sphingobases, such as sphinganine, phytosphinganine, or 4-sphingenine to produce E- and Z-isomers of 8-sphingenine, 8-phytosphingenine, or 4,8-sphingadienine, respectively
689583
Stylosanthes hamata
?
-
-
-
-
phytosphinganine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA8-phytosphingenine + acceptor + H2O
production of two water molecules in the reaction
-
-
?
sphinganine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA8-sphingenine + acceptor + 2 H2O
production of two water molecules in the reaction
-
-
?
Synonyms
Synonyms
Commentary
Organism
DELTA8 sphingobase desaturase
-
Arabidopsis thaliana
DELTA8 sphingobase desaturase
-
Stylosanthes hamata
Delta8 sphingolipid desaturase
-
Arabidopsis thaliana
Delta8 sphingolipid desaturase
-
Stylosanthes hamata
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae confers tolerance to aluminium to the cells
Arabidopsis thaliana
gene S581, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae and constitutively in Arabidopsis thaliana, under control of the CaMV35S promoter, confers tolerance to aluminium and gadolinium, Gd3+, but not to lanthanum, La3+, or manganese, Mn2+, to cells and seedlings, respectively, the transgenic expression leads to the accumulation of 8(Z/E)-C18-phytosphingenine and 8(Z/E)-C20-phytopshingenine in yeast and to the accumulation of 8(Z/E)-C18-phytosphingenine in the leaves and roots of Arabidopsis plants, overview
Stylosanthes hamata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DELTA4-sphingenine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA4,8-sphingadienine + acceptor + 2 H2O
production of two water molecules in the reaction
-
?
phytosphinganine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA8-phytosphingenine + acceptor + H2O
production of two water molecules in the reaction
-
?
sphinganine + reduced acceptor + O2
Stylosanthes hamata
-
DELTA8-sphingenine + acceptor + 2 H2O
production of two water molecules in the reaction
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Stylosanthes hamata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
DELTA4-sphingenine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA4,8-sphingadienine + acceptor + 2 H2O
production of two water molecules in the reaction
-
-
?
additional information
DELTA8 sphingolipid desaturase creates double bonds in long-chain bases, i.e. sphingobases, such as sphinganine, phytosphinganine, or 4-sphingenine to produce E- and Z-isomers of 8-sphingenine, 8-phytosphingenine, or 4,8-sphingadienine, respectively
689583
Stylosanthes hamata
?
-
-
-
-
phytosphinganine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA8-phytosphingenine + acceptor + H2O
production of two water molecules in the reaction
-
-
?
sphinganine + reduced acceptor + O2
-
689583
Stylosanthes hamata
DELTA8-sphingenine + acceptor + 2 H2O
production of two water molecules in the reaction
-
-
?
Other publictions for EC 1.14.19.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746465
Zhou
The sphingolipid biosynthetic ...
Solanum lycopersicum
Sci. Rep.
6
38742
2016
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3
3
1
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746153
Nagano
Arabidopsis Bax inhibitor-1 p ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
240
77-89
2014
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1
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9
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1
2
2
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726865
Li
Newly identified essential ami ...
Brassica rapa
Biochem. Biophys. Res. Commun.
416
165-171
2011
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15
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712836
Habel
Conformational studies on the ...
Helianthus annuus
J. Org. Chem.
75
4975-4982
2010
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1
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699412
Park
An alternate pathway to long-c ...
Papio anubis
J. Lipid Res.
50
1195-1202
2009
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1
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700049
Oura
Disruption of the sphingolipid ...
Candida albicans
Microbiology
154
3795-3803
2008
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1
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689583
Ryan
A higher plant DELTA8 sphingol ...
Arabidopsis thaliana, Stylosanthes hamata
Plant Physiol.
144
1968-1977
2007
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2
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673544
Tripodi
Functional characterization of ...
Leishmania major
FEBS J.
273
271-280
2006
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1
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673639
Sayanova
The alternative pathway C20 DE ...
Acanthamoeba castellanii
FEBS Lett.
580
1946-1952
2006
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1
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658684
Tonon
Fatty acid desaturases from th ...
Thalassiosira pseudonana
FEBS J.
272
3401-3412
2005
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657894
Michaelson
Isolation and characterization ...
Aquilegia vulgaris
Biochem. Soc. Trans.
30
1073-1075
2002
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658490
Takakuwa
Isolation and characterization ...
Kluyveromyces lactis, Lachancea kluyveri
Curr. Microbiol.
45
459-461
2002
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2
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684433
Beckmann Christop
Characterization of a DELTA8-s ...
Helianthus annuus, no activity in Saccharomyces cerevisiae
Angew. Chem. Int. Ed. Engl.
41
2298-2300
2002
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1
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657640
Sperling
Functional identification of a ...
Borago officinalis
Arch. Biochem. Biophys.
388
293-298
2001
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1
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4
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657719
Libisch
Chimeras of DELTA6-fatty acid ...
Borago officinalis
Biochem. Biophys. Res. Commun.
279
779-785
2000
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657892
Sperling
Further characterization of DE ...
Helianthus annuus
Biochem. Soc. Trans.
28
638-641
2000
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659831
Chen
The questionable role of a mic ...
Rattus norvegicus
Lipids
35
871-879
2000
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657637
Wallis
The DELTA8-desaturase of Eugle ...
Euglena gracilis
Arch. Biochem. Biophys.
365
307-316
1999
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