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Literature summary for 1.14.19.4 extracted from

  • Beckmann Christop, B.C.; Rattke Janin, R.J.; Oldham Neil, O.N.; Sperling Petr, S.P.; Heinz Erns, H.E.; Boland Wilhel, B.W.
    Characterization of a DELTA8-sphingolipid desaturase from higher plants: a stereochemical and mechanistic study on the origin of E,Z isomers (2002), Angew. Chem. Int. Ed. Engl., 41, 2298-2300.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in Saccharomyces cerevisiae, using the constitutive ADH1 promotor of the yeast expression vector pVT-U-102, results in synthesis of acylated (8E)- and (8Z)-4-hydroxy-8-sphingenine via N-acyl-4-hydroxysphinganine from fed deuterium-labeled palmitic acid, primary kinetic isotope effects, overview Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus
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no activity in Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phytosphinganine + reduced acceptor + O2 reaction in a in a stereospecific manner by syn elimination of two vicinal hydrogen atoms Helianthus annuus (8E)-4-hydroxy-8-sphingenine + (8Z)-4-hydroxy-8-sphingenine + acceptor + H2O production of two water molecules in the reaction ?

Synonyms

Synonyms Comment Organism
DELTA8-sphingolipid desaturase
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Helianthus annuus