BRENDA - Enzyme Database
show all sequences of 1.14.19.35

Non-redundant contribution of the plastidial FAD8 omega-3 desaturase to glycerolipid unsaturation at different temperatures in Arabidopsis

Roman, A.; Hernandez, M.L.; Soria-Garcia, A.; Lopez-Gomollon, S.; Lagunas, B.; Picorel, R.; Martinez-Rivas, J.M.; Alfonso, M.; Mol. Plant 8, 1599-1611 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene FAD7, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants; gene FAD8, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants. Recombinant expression of AtFAD8-YFP fusion protein in stable transgenic Arabidopsis thaliana lines and transiently in Nicotinana benthamiana leaves, overexpression of YFP-tagged fad8 in Arabidosis thaliana results in increased 18:3 trienoic fatty acid levels in phospholipids but not in galactolipids
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
C103Y
mutation occuring in the fad3 fad7 fad8 triple mutant
Arabidopsis thaliana
additional information
construction of a tandem T-DNA FAD7 insertion omega-3 desaturase mutant line SALK_147096C/fad7i. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable; construction of a tandem T-DNA FAD8 insertion omega-3 desaturase mutant line SALK_093590 with no phenotype at 22°C. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. FAD8-YFP overexpressing lines show a specific increase in 18:3 fatty acids at 22°C. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Disruption of the AtFAD8 gene in mutant fad8i results in trienoic fatty acid levels almost similar to those from wild-type Col-0. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast envelope
enzymes FAD8 and FAD7 might be located in close vicinity in the envelope membrane; enzymes FAD8 and FAD7 might be located in close vicinity in the envelope membrane
Arabidopsis thaliana
9941
-
membrane
;
Arabidopsis thaliana
16020
-
additional information
localization study of recombinantly expressed fluorescence-tagged enzymes, overview; localization study of recombinantly expressed fluorescence-tagged enzymes, overview
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana
-
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana Col-0
-
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana Col-0
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
additional information
Arabidopsis thaliana
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
?
-
-
-
additional information
Arabidopsis thaliana Col-0
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
P46310
gene fad7
-
Arabidopsis thaliana
P48622
gene FAD8
-
Arabidopsis thaliana Col-0
P46310
gene fad7
-
Arabidopsis thaliana Col-0
P48622
gene FAD8
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana Col-0
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana Col-0
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
additional information
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
734740
Arabidopsis thaliana
?
-
-
-
-
additional information
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
734740
Arabidopsis thaliana Col-0
?
-
-
-
-
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
8
30
active at; active at
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
required; required
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene FAD7, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants
Arabidopsis thaliana
gene FAD8, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants. Recombinant expression of AtFAD8-YFP fusion protein in stable transgenic Arabidopsis thaliana lines and transiently in Nicotinana benthamiana leaves, overexpression of YFP-tagged fad8 in Arabidosis thaliana results in increased 18:3 trienoic fatty acid levels in phospholipids but not in galactolipids
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
required
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C103Y
mutation occuring in the fad3 fad7 fad8 triple mutant
Arabidopsis thaliana
additional information
construction of a tandem T-DNA FAD7 insertion omega-3 desaturase mutant line SALK_147096C/fad7i. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable
Arabidopsis thaliana
additional information
construction of a tandem T-DNA FAD8 insertion omega-3 desaturase mutant line SALK_093590 with no phenotype at 22°C. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. FAD8-YFP overexpressing lines show a specific increase in 18:3 fatty acids at 22°C. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Disruption of the AtFAD8 gene in mutant fad8i results in trienoic fatty acid levels almost similar to those from wild-type Col-0. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast envelope
enzymes FAD8 and FAD7 might be located in close vicinity in the envelope membrane
Arabidopsis thaliana
9941
-
membrane
-
Arabidopsis thaliana
16020
-
additional information
localization study of recombinantly expressed fluorescence-tagged enzymes, overview
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana
-
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana Col-0
-
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Arabidopsis thaliana Col-0
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
additional information
Arabidopsis thaliana
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
?
-
-
-
additional information
Arabidopsis thaliana Col-0
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana Col-0
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734740
Arabidopsis thaliana Col-0
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
additional information
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
734740
Arabidopsis thaliana
?
-
-
-
-
additional information
enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied
734740
Arabidopsis thaliana Col-0
?
-
-
-
-
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
8
30
active at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
malfunction
a fad8 mutant shows no significant changes in its fatty acid profile at a control (22°C) temperature, while at lower temperature (15°C) the mutant shows a phenotype, leaf lipid analysis, overview; mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable. After disruption of the AtFAD7 gene, enzyme FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C
Arabidopsis thaliana
additional information
FAD8-YFP over-expressing lines show a specific increase in 18:3 fatty acids at 22°C. Residue 103 is part of the first His box essential for desaturase function
Arabidopsis thaliana
physiological function
plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C; plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C, indicating that enzyme FAD8 is active at this growth temperature, contrasting to previous observations that circumscribe the FAD8 activity at low temperatures
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable. After disruption of the AtFAD7 gene, enzyme FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C
Arabidopsis thaliana
malfunction
a fad8 mutant shows no significant changes in its fatty acid profile at a control (22°C) temperature, while at lower temperature (15°C) the mutant shows a phenotype, leaf lipid analysis, overview
Arabidopsis thaliana
additional information
FAD8-YFP over-expressing lines show a specific increase in 18:3 fatty acids at 22°C. Residue 103 is part of the first His box essential for desaturase function
Arabidopsis thaliana
physiological function
plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C
Arabidopsis thaliana
physiological function
plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C, indicating that enzyme FAD8 is active at this growth temperature, contrasting to previous observations that circumscribe the FAD8 activity at low temperatures
Arabidopsis thaliana
Other publictions for EC 1.14.19.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746056
Bhunia
Enhancement of alpha-linoleni ...
Sesamum indicum, Sesamum indicum Var-9
Plant Cell Rep.
35
213-226
2016
-
1
1
-
1
-
-
-
3
-
-
2
-
5
-
-
-
-
-
1
-
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4
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-
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1
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1
1
1
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1
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3
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2
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1
-
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4
-
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-
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-
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-
-
-
-
-
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-
746058
Lee
Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
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6
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2
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4
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10
-
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-
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-
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-
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746126
Tovuu
Rice mutants deficient in ome ...
Oryza sativa Japonica Group
Plant Physiol. Biochem.
109
525-535
2016
-
-
1
-
1
-
-
-
1
-
-
1
-
3
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
1
2
2
1
-
-
733913
Lim
Threonine 286 of fatty acid de ...
Chlamydomonas reinhardtii
Front. Microbiol.
6
66
2015
-
-
1
-
7
-
-
-
1
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
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-
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1
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7
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1
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1
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-
-
-
-
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-
-
-
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2
2
-
-
-
734740
Roman
Non-redundant contribution of ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Mol. Plant
8
1599-1611
2015
-
-
1
-
2
-
-
-
3
-
-
10
-
15
-
-
-
-
-
1
-
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10
-
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1
-
-
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1
-
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-
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2
2
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3
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6
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10
-
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2
-
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10
-
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2
-
-
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-
-
-
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3
5
-
-
-
745809
Roman
Non-redundant contribution of ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Mol. Plant
8
1599-1611
2015
-
-
-
-
1
-
-
-
2
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
1
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-
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2
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-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
734666
Karabudak
Glycine betaine protects tomat ...
Solanum lycopersicum
Mol. Biol. Rep.
41
1401-1410
2014
-
-
1
-
-
-
1
-
1
-
-
2
-
3
-
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-
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-
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2
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1
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1
-
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1
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2
-
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-
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2
-
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-
-
-
-
-
-
-
1
2
2
1
-
-
743972
Ma
-
Isolation and expression of C ...
Camellia sinensis
Acta Physiol. Plant.
36
2345-2352
2014
-
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
2
-
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2
1
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-
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1
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2
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2
2
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2
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2
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4
-
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2
2
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2
2
1
2
4
-
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734015
Teixeira
omega-3 Fatty acid desaturase ...
Portulaca oleracea
J. Agric. Food Chem.
58
1870-1877
2010
-
-
1
-
-
-
-
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2
-
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2
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2
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1
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2
1
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1
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2
2
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4
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2
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2
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2
2
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2
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734866
Venegas-Caleron
The sunflower plastidial omega ...
Helianthus annuus
Phytochemistry
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2010
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734908
Chaturvedi
Plastid omega3-fatty acid desa ...
Arabidopsis thaliana
Plant J.
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2008
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2
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734953
Andreu
In situ molecular identificati ...
Glycine max
Plant Physiol.
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1336-1344
2007
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732642
Venegas-Caleron
Functional characterization of ...
Helianthus annuus
Plant Physiol. Biochem.
44
517-525
2006
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1
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734153
Matsuda
A temperature-sensitive mechan ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Biol. Chem.
280
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2005
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3
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1
1
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734907
Zhang
Modulated fatty acid desaturat ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant J.
44
361-371
2005
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2
1
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734558
Nishiuchi
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Roles of plastid omega-3 fatty ...
Arabidopsis thaliana, Nicotiana tabacum, Petroselinum crispum, Solanum tuberosum, Zea mays
J. Plant Res.
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481-486
1998
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15
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20
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5
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7
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734559
Kusumi
-
Characterization of a nonsense ...
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111
87-91
1998
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1
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1
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9
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734927
Berberich
Two maize genes encoding omega ...
Zea mays, Zea mays HoneyBantum
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297-306
1998
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1
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6
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734902
Nishiuchi
Wounding changes the spatial e ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell
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1701-1712
1997
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734986
Horiguchi
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Role of omega-3 fatty acid des ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
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1996
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732649
McConn
A Mutation at the fad8 locus o ...
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Kodama
Genetic enhancement of cold to ...
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Gibson
Cloning of a temperature-regul ...
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Iba
A gene encoding a chloroplast ...
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