BRENDA - Enzyme Database
show all sequences of 1.14.19.35

omega-3 Fatty acid desaturase genes isolated from purslane (Portulaca oleracea L.): Expression in different tissues and response to cold and wound stress

Teixeira, M.; Carvalho, I.; Brodelius, M.; J. Agric. Food Chem. 58, 1870-1877 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene fad7, DNA and amino acid sequence determination and analysis; gene fad8, DNA and amino acid sequence determination and analysis
Portulaca oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast membrane
;
Portulaca oleracea
31969
-
additional information
ubiquitous expression, tissue-specific levels; ubiquitous expression, tissue-specific levels
Portulaca oleracea
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Portulaca oleracea
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Portulaca oleracea
A8DTI8
gene fad7
-
Portulaca oleracea
C67ZGG8
gene FAD8
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Portulaca oleracea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734015
Portulaca oleracea
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence; three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
;
Portulaca oleracea
Cloned(Commentary) (protein specific)
Commentary
Organism
gene fad7, DNA and amino acid sequence determination and analysis
Portulaca oleracea
gene fad8, DNA and amino acid sequence determination and analysis
Portulaca oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Portulaca oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast membrane
-
Portulaca oleracea
31969
-
additional information
ubiquitous expression, tissue-specific levels
Portulaca oleracea
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Portulaca oleracea
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Portulaca oleracea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734015
Portulaca oleracea
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Expression
Organism
Commentary
Expression
Portulaca oleracea
FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature; FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up
General Information
General Information
Commentary
Organism
evolution
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence; three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
General Information (protein specific)
General Information
Commentary
Organism
evolution
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Expression (protein specific)
Organism
Commentary
Expression
Portulaca oleracea
FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up
Portulaca oleracea
FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up
Other publictions for EC 1.14.19.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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746058
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Perilla frutescens var. frutescens
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35
2523-2537
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1
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2
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10
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746126
Tovuu
Rice mutants deficient in ome ...
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Plant Physiol. Biochem.
109
525-535
2016
-
-
1
-
1
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1
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1
2
2
1
-
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733913
Lim
Threonine 286 of fatty acid de ...
Chlamydomonas reinhardtii
Front. Microbiol.
6
66
2015
-
-
1
-
7
-
-
-
1
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3
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1
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7
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2
2
-
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-
734740
Roman
Non-redundant contribution of ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Mol. Plant
8
1599-1611
2015
-
-
1
-
2
-
-
-
3
-
-
10
-
15
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
-
-
1
-
-
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2
2
-
3
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6
-
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10
-
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2
-
-
10
-
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2
-
-
-
-
-
-
-
3
5
-
-
-
745809
Roman
Non-redundant contribution of ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Mol. Plant
8
1599-1611
2015
-
-
-
-
1
-
-
-
2
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
1
-
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-
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1
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1
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2
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1
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-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
734666
Karabudak
Glycine betaine protects tomat ...
Solanum lycopersicum
Mol. Biol. Rep.
41
1401-1410
2014
-
-
1
-
-
-
1
-
1
-
-
2
-
3
-
-
-
-
-
-
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2
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2
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1
2
2
1
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743972
Ma
-
Isolation and expression of C ...
Camellia sinensis
Acta Physiol. Plant.
36
2345-2352
2014
-
-
1
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1
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2
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2
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2
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4
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2
2
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2
2
1
2
4
-
-
734015
Teixeira
omega-3 Fatty acid desaturase ...
Portulaca oleracea
J. Agric. Food Chem.
58
1870-1877
2010
-
-
1
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2
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2
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2
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2
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2
2
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1
1
2
2
-
-
734866
Venegas-Caleron
The sunflower plastidial omega ...
Helianthus annuus
Phytochemistry
71
1050-1058
2010
-
-
1
-
1
-
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5
-
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6
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2
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734908
Chaturvedi
Plastid omega3-fatty acid desa ...
Arabidopsis thaliana
Plant J.
54
106-117
2008
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-
1
-
-
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-
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1
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7
-
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2
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2
2
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734953
Andreu
In situ molecular identificati ...
Glycine max
Plant Physiol.
145
1336-1344
2007
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-
-
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4
-
1
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4
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1
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1
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732642
Venegas-Caleron
Functional characterization of ...
Helianthus annuus
Plant Physiol. Biochem.
44
517-525
2006
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1
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3
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1
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5
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4
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1
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1
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734153
Matsuda
A temperature-sensitive mechan ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Biol. Chem.
280
3597-3604
2005
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-
1
-
1
-
1
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2
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17
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2
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3
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1
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1
1
1
1
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734907
Zhang
Modulated fatty acid desaturat ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant J.
44
361-371
2005
-
2
1
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1
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1
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6
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2
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734558
Nishiuchi
-
Roles of plastid omega-3 fatty ...
Arabidopsis thaliana, Nicotiana tabacum, Petroselinum crispum, Solanum tuberosum, Zea mays
J. Plant Res.
111
481-486
1998
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7
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15
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20
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5
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7
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734559
Kusumi
-
Characterization of a nonsense ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Plant Res.
111
87-91
1998
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1
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1
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9
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734927
Berberich
Two maize genes encoding omega ...
Zea mays, Zea mays HoneyBantum
Plant Mol. Biol.
36
297-306
1998
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1
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6
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1
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2
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734902
Nishiuchi
Wounding changes the spatial e ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell
9
1701-1712
1997
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1
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2
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11
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1
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2
2
2
2
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734986
Horiguchi
-
Role of omega-3 fatty acid des ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
199
439-442
1996
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1
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1
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2
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9
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2
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732649
McConn
A Mutation at the fad8 locus o ...
Arabidopsis thaliana
Plant Physiol.
106
1609-1614
1994
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Genetic enhancement of cold to ...
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1994
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1
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Iba
A gene encoding a chloroplast ...
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1
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1
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1
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1
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1
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1
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