BRENDA - Enzyme Database show
show all sequences of 1.14.19.34

Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity

Dyer, J.M.; Chapital, D.C.; Kuan, J.C.; Mullen, R.T.; Turner, C.; McKeon, T.A.; Pepperman, A.B.; Plant Physiol. 130, 2027-2038 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vernicia fordii
Q8GZC3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.33
732650
Vernicia fordii
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Vernicia fordii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Vernicia fordii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.33
732650
Vernicia fordii
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the FAD2 enzyme family
Vernicia fordii
physiological function
FAD2 converts oleic acid into linoleic acid in the plant seed oil
Vernicia fordii
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the FAD2 enzyme family
Vernicia fordii
physiological function
FAD2 converts oleic acid into linoleic acid in the plant seed oil
Vernicia fordii
Other publictions for EC 1.14.19.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734991
Mietkiewska
Combined transgenic expression ...
Punica granatum
Planta
240
575-583
2014
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-
1
-
-
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-
-
-
-
-
-
1
-
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1
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1
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1
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-
734145
Cahoon
Dimorphecolic acid is synthesi ...
Dimorphotheca sinuata
J. Biol. Chem.
279
12495-12502
2004
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-
1
-
-
-
-
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1
-
4
-
7
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1
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1
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5
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1
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2
1
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1
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4
-
-
-
-
-
2
-
-
5
-
-
-
-
-
-
-
-
-
-
3
6
-
-
-
732650
Dyer
Molecular analysis of a bifunc ...
Vernicia fordii
Plant Physiol.
130
2027-2038
2002
-
-
1
-
-
-
-
-
1
-
-
1
-
1
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2
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1
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1
1
-
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-
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1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
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-