Cloned (Comment) | Organism |
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gene FADX, combined transgenic expression of Punica granatum conjugase (FADX) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase, EC 1.14.19.34, in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis. Arabidopsis thaliana seeds overexpressing PgFADX show accumulation of punicic acid up to 9.2% average in tandem lines (6.43% in lines expressing FADX alone), highest content in line NCJ-11 is 11.26%, phenotypes, overview. Overexpression of PgFADX affects the activity of the native Arabidopsis thaliana FAD2 desaturase, the relative AtFAD2 expression level is significantly reduced up to 99% | Punica granatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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additional information | Punica granatum | linoleic acid serves as a precursor for punicic acid synthesis by the enzyme | ? | - |
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Organism | UniProt | Comment | Textmining |
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Punica granatum | Q84UB8 | PgFADX; gene FADX | - |
Source Tissue | Comment | Organism | Textmining |
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seed | - |
Punica granatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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additional information | linoleic acid serves as a precursor for punicic acid synthesis by the enzyme | Punica granatum | ? | - |
? | |
additional information | the enzyme catalyzes the conversion of the DELTA12-double bond of linoleic acid (18:2DELTA9cis,12cis) into conjugated DELTA11trans and DELTA13cis-double bonds | Punica granatum | ? | - |
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Synonyms | Comment | Organism |
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FADX | - |
Punica granatum |