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Literature summary for 1.14.19.33 extracted from

  • Dyer, J.M.; Chapital, D.C.; Kuan, J.C.; Mullen, R.T.; Turner, C.; McKeon, T.A.; Pepperman, A.B.
    Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity (2002), Plant Physiol., 130, 2027-2038.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Vernicia fordii
gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells Vernicia fordii

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Vernicia fordii 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ Vernicia fordii the enzyme is involved in the biosynthetic pathway of eleostearic acid an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate ?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ Vernicia fordii
-
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ Vernicia fordii the enzyme is involved in the biosynthetic pathway of eleostearic acid an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate ?

Organism

Organism UniProt Comment Textmining
Vernicia fordii Q8GZC2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed expressed exclusively within developing seeds Vernicia fordii
-
seed FADX is expressed exclusively in developing tung seeds Vernicia fordii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii (6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
?
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii (9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
?
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii (9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate ?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ the enzyme is involved in the biosynthetic pathway of eleostearic acid Vernicia fordii an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate ?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
Vernicia fordii an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate ?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ the enzyme is involved in the biosynthetic pathway of eleostearic acid Vernicia fordii an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate ?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration Vernicia fordii an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
?
additional information the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates Vernicia fordii ?
-
?
additional information a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34 Vernicia fordii ?
-
?

Synonyms

Synonyms Comment Organism
DELTA12 desaturase
-
Vernicia fordii
DELTA12 oleate desaturase
-
Vernicia fordii
FADX
-
Vernicia fordii

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Vernicia fordii

General Information

General Information Comment Organism
evolution tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity Vernicia fordii
additional information incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions Vernicia fordii
physiological function FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil Vernicia fordii