BRENDA - Enzyme Database
show all sequences of 1.14.19.33

Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity

Dyer, J.M.; Chapital, D.C.; Kuan, J.C.; Mullen, R.T.; Turner, C.; McKeon, T.A.; Pepperman, A.B.; Plant Physiol. 130, 2027-2038 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae; gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vernicia fordii
Q8GZC2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
expressed exclusively within developing seeds; FADX is expressed exclusively in developing tung seeds
Vernicia fordii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates
732650
Vernicia fordii
?
-
-
-
-
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34
732650
Vernicia fordii
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Vernicia fordii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae; gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
Vernicia fordii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Vernicia fordii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Vernicia fordii
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
-
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Vernicia fordii
the enzyme is involved in the biosynthetic pathway of eleostearic acid
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
expressed exclusively within developing seeds; FADX is expressed exclusively in developing tung seeds
Vernicia fordii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
(9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the enzyme is involved in the biosynthetic pathway of eleostearic acid
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration
732650
Vernicia fordii
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates
732650
Vernicia fordii
?
-
-
-
-
additional information
a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34
732650
Vernicia fordii
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity
Vernicia fordii
additional information
incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions
Vernicia fordii
physiological function
FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil
Vernicia fordii
General Information (protein specific)
General Information
Commentary
Organism
evolution
tung FADX is grouped with DELTA12 fatty acid desaturases and hydroxylases rather than conjugases, which is consistent with its desaturase activity
Vernicia fordii
additional information
incubation of yeast cells expressing FADX with exogenously supplied palmitoleicacid leads to substantial incorporation of thisfatty acid into yeast cells, accounting for approximately79% of total fatty acids. Oleic acid is reduced to only 3% under these growth conditions
Vernicia fordii
physiological function
FADX converts linoleic acid into alpha-eleostearic acid. in the plant seed oil
Vernicia fordii
Other publictions for EC 1.14.19.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734991
Mietkiewska
Combined transgenic expression ...
Punica granatum
Planta
240
575-583
2014
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1
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1
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1
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1
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2
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1
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1
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1
-
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2
-
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-
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-
-
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-
-
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-
734237
Okada
Diversity of DELTA12 fatty aci ...
Exocarpos cupressiformis, Santalum acuminatum
J. Biol. Chem.
288
32405-32413
2013
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-
2
-
-
-
-
-
2
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4
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4
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2
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8
-
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-
-
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-
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-
-
-
-
-
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4
-
-
-
-
-
-
-
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4
-
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4
-
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-
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4
-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
734176
Rawat
Conjugated fatty acid synthesi ...
Momordica charantia
J. Biol. Chem.
287
16230-16237
2012
-
-
1
-
4
-
-
-
-
-
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1
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-
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1
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1
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4
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-
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-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732650
Dyer
Molecular analysis of a bifunc ...
Vernicia fordii
Plant Physiol.
130
2027-2038
2002
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
1
-
-
11
-
-
-
-
-
-
-
-
1
-
-
-
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1
1
-
-
-
-
-
-
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1
-
-
3
-
-
-
-
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
732839
Cahoon
Biosynthetic origin of conjuga ...
Impatiens balsamina, Momordica charantia
Proc. Natl. Acad. Sci. USA
96
12935-12940
1999
-
-
2
-
-
-
-
-
-
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4
-
2
-
-
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2
-
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4
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2
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4
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4
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