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Literature summary for 1.14.19.3 extracted from
- Effect of two intermediate electron donors, NADPH and FADH(2), on Spirulina DELTA6-desaturase co-expressed with two different immediate electron donors, cytochrome b5 and ferredoxin, in Escherichia coli (2006), Mol. Biol. Rep., 34, 261-266.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha, co-expressed with cytochrome b5 and ferredoxin, no enzyme activity when expressed in Escherichia coli alone | Arthrospira platensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
Western Blot, detection of histidine-tagged DELTA6 desaturase expressed in Escherichia coli using a monoclonal antibody against the histidine-tag | Arthrospira platensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrospira platensis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.3 mM exogenously added NADPH to Escherichia coli cells co-expressing DELTA6 desaturase and cytochrome b5 leads to an increase of the level of gamma-linoleic acid product by 50%, but no effect in Escherichia coli co-expressing DELTA6 desaturase and ferredoxin under the same conditions | Arthrospira platensis |
| additional information | - |
co-addition of NADPH and FAD to Escherichia coli cells co-expressing DELTA6 desaturase and ferredoxin leads to an increase of the level of gamma-linoleic acid product | Arthrospira platensis |
| additional information | - |
no activity when expressed in Escherichia coli alone, co-expression of cytochrome b5 is needed | Arthrospira platensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleic acid + AH2 + O2 | - |
Arthrospira platensis | gamma-linolenic acid + A + H2O | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA6 desaturase | - |
Arthrospira platensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | with NADPH | Arthrospira platensis | |
| Ferredoxin | with FADH2 | Arthrospira platensis |
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