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Literature summary for 1.14.19.3 extracted from

  • Kurdrid, P.; Subudhi, S.; Hongsthong, A.; Ruengjitchatchawalya, M.; Tanticharoen, M.
    Functional expression of Spirulina-DELTA6 desaturase gene in yeast, Saccharomyces cerevisiae (2005), Mol. Biol. Rep., 32, 215-226.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae, strain DBY746 with and without supplementation with linoleic acid Arthrospira platensis

Protein Variants

Protein Variants Comment Organism
H129G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
H129R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate Arthrospira platensis
H305G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
H305R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate Arthrospira platensis
H89G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
H89R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity using linoleic acid as substrate Arthrospira platensis

Organism

Organism UniProt Comment Textmining
Arthrospira platensis
-
C1
-
Arthrospira platensis C1
-
C1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + AH2 + O2
-
Arthrospira platensis gamma-linolenic acid + A + H2O
-
?
linoleic acid + AH2 + O2
-
Arthrospira platensis C1 gamma-linolenic acid + A + H2O
-
?