BRENDA - Reference to 1.14.19.3; Id = 438414

NADH-cytochrome b5 reductase

EC 1.6.2.2, absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADPH-cytochrome b5 reductase

EC 1.6.2.2, absolutely essential for DELTA6-desaturation

Rattus norvegicus

O2

absolutely essential for DELTA6-desaturation

Rattus norvegicus

bathophenanthroline sulfonate

mild inhibition

Rattus norvegicus

beta-mercaptoethanol

mild inhibition

Rattus norvegicus

dithiothreitol

mild inhibition

Rattus norvegicus

iron chelators

-

Rattus norvegicus

KCN

-

Rattus norvegicus

N-ethylmaleimide

only little effect

Rattus norvegicus

p-chloromercuribenzene sulfonate

-

Rattus norvegicus

Tiron

-

Rattus norvegicus

0.045

-

linoleoyl-CoA

-

Rattus norvegicus

microsome

membrane

Rattus norvegicus

-

Fe2+

DELTA6-desaturase contains 15.1 nmol iron/mg protein

Rattus norvegicus

65000

68000

gel filtration

Rattus norvegicus

66000

-

SDS-PAGE

Rattus norvegicus

linoleic acid + AH2 + O2

Rattus norvegicus

-

gamma-linolenic acid + A + H2O

-

?

linoleic acid + AH2 + O2

Rattus norvegicus Wistar

-

gamma-linolenic acid + A + H2O

-

?

Rattus norvegicus

-

Rattus norvegicus Wistar

-

phospholipoprotein

20-40 M phospholipid per mol of protein

Rattus norvegicus

-

Rattus norvegicus

liver

-

Rattus norvegicus

-

0.0344

-

Rattus norvegicus

-70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing

Rattus norvegicus

linoleic acid + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenic acid + A + H2O

-

?

linoleic acid + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenic acid + A + H2O

-

?

linoleoyl-CoA + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenoyl-CoA + A + 2 H2O

-

linoleoyl-CoA + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenoyl-CoA + A + 2 H2O

-

octadeca-9,12-dienoic acid + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenic acid + A + H2O

-

438414

Rattus norvegicus

?

octadeca-9,12-dienoic acid + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenic acid + A + H2O

-

438414

Rattus norvegicus Wistar

?

monomer

1 * 66000, SDS-PAGE

Rattus norvegicus

7

-

Rattus norvegicus

6

9

-

Rattus norvegicus

cytochrome b5

absolutely essential for DELTA6-desaturation

Rattus norvegicus

linoleoyl-CoA

absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADH

absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADPH

absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADH-cytochrome b5 reductase

EC 1.6.2.2, absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADPH-cytochrome b5 reductase

EC 1.6.2.2, absolutely essential for DELTA6-desaturation

Rattus norvegicus

O2

absolutely essential for DELTA6-desaturation

Rattus norvegicus

cytochrome b5

absolutely essential for DELTA6-desaturation

Rattus norvegicus

linoleoyl-CoA

absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADH

absolutely essential for DELTA6-desaturation

Rattus norvegicus

NADPH

absolutely essential for DELTA6-desaturation

Rattus norvegicus

bathophenanthroline sulfonate

mild inhibition

Rattus norvegicus

beta-mercaptoethanol

mild inhibition

Rattus norvegicus

dithiothreitol

mild inhibition

Rattus norvegicus

iron chelators

-

Rattus norvegicus

KCN

-

Rattus norvegicus

N-ethylmaleimide

only little effect

Rattus norvegicus

p-chloromercuribenzene sulfonate

-

Rattus norvegicus

Tiron

-

Rattus norvegicus

0.045

-

linoleoyl-CoA

-

Rattus norvegicus

microsome

membrane

Rattus norvegicus

-

Fe2+

DELTA6-desaturase contains 15.1 nmol iron/mg protein

Rattus norvegicus

65000

68000

gel filtration

Rattus norvegicus

66000

-

SDS-PAGE

Rattus norvegicus

linoleic acid + AH2 + O2

Rattus norvegicus

-

gamma-linolenic acid + A + H2O

-

?

linoleic acid + AH2 + O2

Rattus norvegicus Wistar

-

gamma-linolenic acid + A + H2O

-

?

phospholipoprotein

20-40 M phospholipid per mol of protein

Rattus norvegicus

-

Rattus norvegicus

liver

-

Rattus norvegicus

-

0.0344

-

Rattus norvegicus

-70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing

Rattus norvegicus

linoleic acid + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenic acid + A + H2O

-

?

linoleic acid + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenic acid + A + H2O

-

?

linoleoyl-CoA + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenoyl-CoA + A + 2 H2O

-

linoleoyl-CoA + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenoyl-CoA + A + 2 H2O

-

octadeca-9,12-dienoic acid + AH2 + O2

-

438414

Rattus norvegicus

gamma-linolenic acid + A + H2O

-

438414

Rattus norvegicus

?

octadeca-9,12-dienoic acid + AH2 + O2

-

438414

Rattus norvegicus Wistar

gamma-linolenic acid + A + H2O

-

438414

Rattus norvegicus Wistar

?

monomer

1 * 66000, SDS-PAGE

Rattus norvegicus

7

-

Rattus norvegicus

6

9

-

Rattus norvegicus

744584

Jiang

Functional identification and ...

Mucor sp., Mucor sp. EIM-10

Biotechnol. Lett.

39

453-461

2017

-

1

-

1

-

1

-

2

-

2

-

2

-

2

-

1

-

1

-

1

-

1

-

2

-

2

-

745488

Yary

Omega-6 polyunsaturated fatty ...

Homo sapiens

J. Hum. Nutr. Diet.

30

506-514

2017

-

1

-

2

-

1

-

2

-

1

-

1

-

745520

Shi

Molecular mechanisms underlyi ...

Glossomastix chrysoplasta, Thalassiosira pseudonana

J. Lipid Res.

59

79-88

2017

-

2

-

22

-

2

-

4

-

2

-

3

-

6

-

7

-

2

-

22

-

2

-

4

-

6

-

4

-

744420

Park

Palmitic acid (160) competes ...

Papio anubis

Biochim. Biophys. Acta

1861

91-97

2016

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

1

-

744747

Geay

Cloning and functional charac ...

Perca fluviatilis

Comp. Biochem. Physiol. B

191

112-125

2016

-

1

-

1

-

4

-

4

-

3

-

1

-

1

-

1

-

4

-

2

-

745516

Vaittinen

FADS2 genotype regulates delt ...

Homo sapiens

J. Lipid Res.

57

56-65

2016

-

1

-

2

-

2

-

3

-

1

-

2

-

1

-

745517

Watanabe

Identification of amino acid ...

Rattus norvegicus

J. Lipid Res.

57

89-99

2016

-

1

-

26

-

1

-

1

-

2

-

1

-

26

-

1

-

745702

Shi

Application of a delta-6 desa ...

Micromonas pusilla, Micromonas pusilla CCMP 1545, Mortierella alpina, Mortierella alpina ATCC 32222

Microb. Cell Fact.

15

117

2016

-

1

-

1

-

2

-

10

-

10

-

2

-

10

-

8

-

2

-

1

2

-

1

-

2

-

10

-

2

-

10

-

746355

Nagano

Delta-6 desaturase activity d ...

Homo sapiens

Prostaglandins Leukot. Essent. Fatty Acids

115

8-11

2016

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

744750

Rezapour-Firouzi

Alteration of delta-6-desatur ...

Homo sapiens

Complement. Ther. Med.

23

652-657

2015

-

1

-

2

-

3

-

2

-

2

-

4

-

1

-

2

-

2

-

2

-

2

-

745514

Shi

Molecular mechanism of substr ...

Micromonas pusilla, Micromonas pusilla CCMP 1545, Mortierella alpina, Mortierella alpina ATCC 32222

J. Lipid Res.

56

2309-2321

2015

-

2

-

9

-

2

-

12

-

7

-

20

-

9

2

-

2

-

2

-

9

-

2

-

12

-

20

-

2

-

4

-

728540

Song

Identification of the substrat ...

Ribes nigrum

Planta

239

753-763

2014

-

1

-

1

-

2

-

2

-

1

-

2

-

743973

Shokri

-

Delta-6 desaturase makes a di ...

Echium amoenum

Acta Physiol. Plant.

36

2413-2418

2014

-

1

-

2

-

1

-

1

-

2

1

4

-

1

-

1

-

2

-

1

-

2

1

-

3

-

744645

Kim

-

Functional characterization o ...

Acanthopagrus schlegelii

Cell Biochem. Biophys.

68

335-346

2014

-

1

-

2

-

1

-

2

-

3

-

1

-

1

-

2

-

2

-

2

-

744947

Wang

Transgenic expression of salm ...

Salmo salar

Fish Shellfish Immunol.

39

223-230

2014

-

1

-

5

-

3

-

2

-

1

-

3

-

745049

Lopez-Vicario

Molecular interplay between D ...

Mus musculus

Gut

63

344-355

2014

-

1

-

1

-

1

-

1

-

1

-

726698

Lu

The thermostability of two kin ...

Rhizopus stolonifer, Rhizopus stolonifer R31.6

Appl. Biochem. Biotechnol.

171

165-172

2013

-

1

-

1

-

2

-

1

-

1

4

1

-

1

-

1

-

1

-

1

-

1

4

1

-

1

-

727270

Kim

Functional characterization of ...

Acanthopagrus schlegelii

Cell Biochem. Biophys.

68

335-346

2013

-

1

-

1

-

6

-

2

-

3

1

3

-

1

-

1

-

2

-

3

1

-

727338

Tanomman

Characterization of fatty acid ...

Oreochromis niloticus

Comp. Biochem. Physiol. B

166

148-156

2013

-

1

-

3

-

5

-

2

-

2

-

1

-

5

-

2

-

727393

Dahmen

Cytochrome b5 coexpression inc ...

Tetrahymena thermophila, Tetrahymena thermophila ATCC 30007

Eukaryot. Cell

12

923-931

2013

-

1

-

2

-

2

-

2

-

1

-

1

-

2

-

728113

Kim

Isolation and functional chara ...

Muraenesox cinereus, Muraenesox cinereus pike eel

J. Microbiol.

51

807-813

2013

-

1

-

4

-

1

-

4

-

3

-

1

-

1

-

4

-

1

-

734875

Sayanova

The role of DELTA6-desaturase ...

Mus musculus, Ostreococcus tauri, Salmo salar

Plant Biotechnol. J.

10

195-206

2012

-

3

-

6

-

3

-

6

-

6

-

3

-

3

-

6

-

6

-

713932

Vagner

-

Characterization and modulatio ...

Cyprinus carpio, Danio rerio, Dicentrarchus labrax, Gadus morhua, Oncorhynchus mykiss, Rachycentron canadum, Salmo salar, Scophthalmus maximus, Sparus aurata

Aquaculture

315

131-143

2011

-

1

-

9

-

1

-

9

-

1

-

1

-

18

-

714577

Kim

Functional characterization of ...

Acanthopagrus schlegelii

Biotechnol. Lett.

33

1185-1193

2011

-

1

-

5

-

2

-

3

-

1

-

2

-

715397

Reisner

Trans fat diet causes decrease ...

Caenorhabditis elegans

J. Biochem. Mol. Toxicol.

25

269-279

2011

-

1

-

4

-

1

-

2

-

1

-

1

-

2

-

716087

Santigosa

Cloning, tissue expression ana ...

Dicentrarchus labrax

Mar. Biotechnol.

13

22-31

2011

-

1

-

6

-

4

-

2

-

726857

Chen

Delta-6 desaturase from borage ...

Borago officinalis

Biochem. Biophys. Res. Commun.

410

484-488

2011

-

1

-

2

-

1

-

1

-

1

-

1

-

714315

Monroig

Multiple genes for functional ...

Salmo salar

Biochim. Biophys. Acta

1801

1072-1081

2010

-

1

-

8

-

1

-

2

-

4

-

3

-

3

-

2

-

1

3

-

716085

Iskandarov

Identification and characteriz ...

Lobosphaera incisa

Lipids

45

519-530

2010

-

1

-

1

-

1

-

4

-

1

-

2

-

1

-

1

-

1

-

1

-

716909

Huang

-

Cloning and functional charact ...

Oenothera biennis

Russ. J. Plant Physiol.

57

568-573

2010

-

1

-

2

-

1

-

2

1

2

-

1

-

1

-

2

-

2

1

-

1

-

699496

Wan

Production of gamma-linolenic ...

Cunninghamella echinulata, Cunninghamella echinulata MIAN6

J. Microbiol. Biotechnol.

19

1098-1102

2009

-

1

-

1

-

7

-

2

-

1

-

2

-

1

2

-

1

-

2

-

700115

Lu

Identification and characteriz ...

Rhizopus stolonifer

Mol. Biol. Rep.

36

2291-2297

2009

-

1

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

2

-

1

-

684884

Subudhi

Isolation and functional chara ...

Arthrospira platensis, Arthrospira platensis C1

Biochem. Biophys. Res. Commun.

365

643-649

2008

-

1

-

5

-

2

-

1

-

697459

Li

The effects of dietary fatty a ...

Siganus canaliculatus

Comp. Biochem. Physiol. B

151

183-190

2008

-

3

-

3

-

2

-

1

-

3

-

2

-

675119

Wang

Identification and functional ...

Thamnidium elegans, Thamnidium elegans As.3.2806, Thamnidium elegans As3.2806

J. Eukaryot. Microbiol.

54

110-117

2007

-

1

-

7

-

1

-

2

1

-

2

-

1

-

2

1

-

675761

Hsiao

Identification and functional ...

Glossomastix chrysoplasta

Mar. Biotechnol.

9

154-165

2007

-

1

-

5

-

2

-

1

-

1

-

2

-

689837

Das

A defect in the activity of DE ...

Homo sapiens

Prostaglandins Leukot. Essent. Fatty Acids

76

251-268

2007

-

1

-

1

-

3

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

3

-

1

-

1

-

2

-

675898

Kurdrid

Effect of two intermediate ele ...

Arthrospira platensis

Mol. Biol. Rep.

34

261-266

2006

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

2

-

1

-

1

-

1

-

676702

Eckert

Co-expression of the borage DE ...

Borago officinalis

Planta

224

1050-1057

2006

-

1

-

1

-

1

-

657868

Domergue

In vivo characterization of th ...

Ostreococcus tauri

Biochem. J.

389

483-490

2005

-

1

-

1

-

7

-

1

-

2

-

1

-

2

1

-

1

-

1

-

658309

Abe

Identification of mutation sit ...

Mortierella alpina

Biosci. Biotechnol. Biochem.

69

1021-1024

2005

-

3

-

2

-

1

-

3

-

658754

Na-Ranong

Substrate specificity and pref ...

Amylomyces rouxii

FEBS Lett.

579

2744-2748

2005

-

1

-

4

-

5

-

1

-

1

-

5

-

659836

Zheng

Highly unsaturated fatty acid ...

Salmo salar

Lipids

40

13-24

2005

-

1

-

5

-

9

-

1

-

9

-

675896

Kurdrid

Functional expression of Spiru ...

Arthrospira platensis, Arthrospira platensis C1

Mol. Biol. Rep.

32

215-226

2005

-

1

-

6

-

2

-

2

-

1

-

6

-

2

-

657900

Guillou

The surprising diversity of DE ...

Rattus norvegicus

Biochem. Soc. Trans.

32

86-87

2004

-

1

-

1

-

2

-

2

-

1

-

2

-

658475

Zheng

Characterization and compariso ...

Cyprinus carpio, Oncorhynchus mykiss, Scophthalmus maximus, Sparus aurata

Comp. Biochem. Physiol. B

139

269-279

2004

-

4

-

8

-

13

-

2

-

16

-

4

-

4

-

8

-

2

-

16

-

660486

Jan

Myristic acid increases DELTA6 ...

Rattus norvegicus

Reprod. Nutr. Dev.

44

131-140

2004

-

2

-

3

-

3

-

3

-

3

-

658248

Saether

Expression and regulation of D ...

Rattus norvegicus

Biol. Reprod.

69

117-124

2003

-

2

-

5

-

5

-

658300

Sakuradani

Gene cloning and functional an ...

Mortierella alpina

Biosci. Biotechnol. Biochem.

67

704-711

2003

-

1

-

7

-

1

-

1

-

658711

Sayanova

Identification of primula fatt ...

Primula farinosa, Primula vialii

FEBS Lett.

542

100-104

2003

-

2

-

2

-

2

-

8

-

2

-

2

-

2

-

8

-

659649

Ge

Identification of the DELTA-6 ...

Homo sapiens

J. Invest. Dermatol.

120

707-714

2003

-

1

-

3

-

3

-

2

-

3

-

1

-

3

-

2

-

438422

D'Andrea

The same rat DELTA6-desaturase ...

Homo sapiens, Rattus norvegicus, Rattus norvegicus Sprague-Dawley

Biochem. J.

364

49-55

2002

-

2

-

2

-

1

3

-

165

-

4

-

13

-

2

-

2

-

1

3

-

4

-

13

-

438423

Ramanadham

DELTA6-, stearoyl CoA-, and DE ...

Homo sapiens, Rattus norvegicus

Biochim. Biophys. Acta

1580

40-56

2002

-

2

-

2

-

2

-

6

-

2

-

2

-

6

-

438420

Chuang

Effect of conjugated linoleic ...

Mortierella alpina

Lipids

36

139-143

2001

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

438421

Williard

Identification of a fatty acid ...

Homo sapiens, Mus musculus, Rattus norvegicus

J. Lipid Res.

42

501-508

2001

-

3

-

4

-

6

-

5

-

3

-

6

-

5

-

438419

Brown

Linoleic acid metabolism in th ...

Homo sapiens, Rattus norvegicus

Lipids

35

1319-1323

2000

-

2

-

2

-

6

-

2

-

4

-

2

-

2

-

2

-

4

-

438443

Schultz

Stearoyl-acyl carrier protein ...

Thunbergia alata

Plant Physiol.

124

681-692

2000

-

1

-

1

-

1

-

438418

Rodriguez

Differential effect of N-ethyl ...

Homo sapiens, Rattus norvegicus

Lipids

34

23-30

1999

-

2

-

3

-

2

-

3

-

6

-

6

-

2

-

3

-

2

-

6

-

6

-

734903

Girke

Identification of a novel DELT ...

Physcomitrella patens

Plant J.

15

39-48

1998

-

1

-

1

-

4

-

1

-

2

-

1

-

1

-

1

-

1

-

438436

Cahoon

Redesign of soluble fatty acid ...

Thunbergia alata

Proc. Natl. Acad. Sci. USA

94

4872-4877

1997

-

1

-

4

-

1

-

1

-

1

-

2

-

1

-

1

-

4

-

1

-

1

-

2

-

438437

Cahoon

Characterization of a structur ...

Thunbergia alata

Plant Mol. Biol.

33

1105-1110

1997

-

1

-

1

-

1

-

1

-

1

-

1

-

438417

Norman

-

In vitro desaturation of monog ...

Arabidopsis thaliana, Arabidopsis thaliana Heynh., Glycine max, Spinacia oleracea

Phytochemistry

30

2217-2222

1991

2

-

2

-

3

-

4

-

4

-

2

-

2

-

10

-

2

-

2

-

3

-

4

-

2

-

2

-

10

-

438416

Griffiths

DELTA 6- and DELTA 12-desatura ...

Borago officinalis

Biochem. J.

252

641-647

1988

-

1

-

1

-

2

-

2

-

3

-

1

-

1

-

2

-

3

-

438415

Garda

Short chain aliphatic alcohols ...

Rattus norvegicus, Rattus norvegicus Wistar

Biochim. Biophys. Acta

769

160-170

1984

-

1

-

2

-

168

-

1

-

4

-

1

-

2

-

1

-

4

-

438412

Browse

-

Catalase stimulates linoleate ...

Linum usitatissimum, Spinacia oleracea

FEBS Lett.

131

111-114

1981

1

-

2

-

2

-

2

-

3

-

4

-

1

-

2

-

2

-

3

-

4

-

438413

Mahfouz

Inhibition of desaturation of ...

Rattus norvegicus

Biochim. Biophys. Acta

663

58-68

1981

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

438414

Okayasu

Purification and partial chara ...

Rattus norvegicus, Rattus norvegicus Wistar

Arch. Biochem. Biophys.

206

21-28

1981

3

-

8

1

2

-

169

-

1

-

2

1

6

1

-

1

-

4

-

3

-

4

-

8

-

1

2

-

1

-

2

1

6

1

-

1

-

438411

Jeffcoat

Evidence for the different res ...

Rattus norvegicus

Biochim. Biophys. Acta

528

28-35

1978

2

-

2

-

1

-

3

-

1

-

2

-

2

-

2

-

1

-

1

-

2

-

Purification and partial characterization of linoleoyl-CoA desaturase from rat liver microsomes

Data extracted from this reference: