Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain INVScI | Brassica rapa |
Protein Variants | Comment | Organism |
---|---|---|
D208R | inactive | Brassica rapa |
D210R | inactive | Brassica rapa |
F59R | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
G28R | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
G368R | inactive | Brassica rapa |
G52R | the mutant shows wild type activity | Brassica rapa |
H63R | inactive | Brassica rapa |
L369A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
L71R | the mutant shows wild type activity | Brassica rapa |
N203R | inactive | Brassica rapa |
Q372H | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
T56R | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
W190A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
W345A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
Y31A | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Brassica rapa | 5783 | - |
membrane | - |
Brassica rapa | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa | - |
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa | - |
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa | G9I7G1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa | (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa | (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D8A | - |
Brassica rapa |
DELTA8-sphingolipid desaturase | - |
Brassica rapa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Brassica rapa |