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Literature summary for 1.14.19.29 extracted from

  • Li, S.F.; Song, L.Y.; Zhang, G.J.; Yin, W.B.; Chen, Y.H.; Wang, R.R.; Hu, Z.M.
    Newly identified essential amino acid residues affecting DELTA8-sphingolipid desaturase activity revealed by site-directed mutagenesis (2011), Biochem. Biophys. Res. Commun., 416, 165-171.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain INVScI Brassica rapa

Protein Variants

Protein Variants Comment Organism
D208R inactive Brassica rapa
D210R inactive Brassica rapa
F59R the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
G28R the mutant shows reduced activity compared to the wild type enzyme Brassica rapa
G368R inactive Brassica rapa
G52R the mutant shows wild type activity Brassica rapa
H63R inactive Brassica rapa
L369A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
L71R the mutant shows wild type activity Brassica rapa
N203R inactive Brassica rapa
Q372H the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
T56R the mutant shows reduced activity compared to the wild type enzyme Brassica rapa
W190A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
W345A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
Y31A the mutant shows reduced activity compared to the wild type enzyme Brassica rapa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Brassica rapa 5783
-
membrane
-
Brassica rapa 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa
-
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa
-
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Brassica rapa G9I7G1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?

Synonyms

Synonyms Comment Organism
D8A
-
Brassica rapa
DELTA8-sphingolipid desaturase
-
Brassica rapa

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Brassica rapa