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Literature summary for 1.14.19.28 extracted from

  • Woelke, M.R.; Paulucci, N.S.; Selva, A.; Garban, H.; de Lema, M.G.
    DELTA9 desaturase from Trypanosoma cruzi key enzyme in the parasite metabolism. Cloning and overexpression (2017), Microbiol. Res., 194, 29-37 .
    View publication on PubMed
Search for more literature for 1.14.19.28

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
ketoconazole
-
 Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Trypanosoma cruzi 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Trypanosoma cruzi
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Trypanosoma cruzi Tulahuen
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi G8FL60
-
-
Trypanosoma cruzi Tulahuen G8FL60
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from epimastigotes partially by ultracentrifugation, recombinant His6-tagged enzyme from Escherichia coli strain BL21 codon Plus cell-free extract by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Trypanosoma cruzi 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Trypanosoma cruzi Tulahuen 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, about, recombinant enzyme, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
9des
-
 Trypanosoma cruzi
DELTA9 desaturase
-
 Trypanosoma cruzi
TcDELTA9DES
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 in vivo assay at Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi about, isoelectric focusing
-
 9

General Information

General Information Comment Organism
metabolism desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. This DELTA9-desaturation reaction normally requires molecular oxygen and reducing equivalents derived from NAD(P)H through an electron system consisting of either NADPH, cytochrome b5 and cytochrome b5-reductase in animals and fungi or ferredoxin-NADP+ oxidoreductase and ferredoxin in bacteria and plants Trypanosoma cruzi
physiological function the enzyme plays a critical role in the metabolism of the parasite Trypanosoma cruzi