BRENDA - Enzyme Database
show all sequences of 1.14.19.25

Heterologous expression of Brassica juncea microsomal omega-3 desaturase gene (BjFad3) improves the nutritionally desirable omega-6omega-3 fatty acid ratio in rice bran oil

Bhattacharya, S.; Chattopadhyaya, B.; Koduru, L.; Das, N.; Maiti, M.; Plant Cell Tissue Organ. Cult. 119, 117-129 (2014)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene fad3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant plamid pCAM::Nap-BjFad3-NOS is introduced into Agrobacterium tumefaciens strain LBA4404/VirGN54D by freeze-thaw method and then used for transformation of Escherichia coli strain DH10B and of Nicotiana tabacum explant, recombinant ectopic expression analysis in leaves of transgenic plants, recombinant expression of the enzyme in transgenic Oryza sativa plants, semi-quantitative RT-PCR expression analysis
Brassica juncea
Engineering
Protein Variants
Commentary
Organism
additional information
seed fatty acid profiles of tobacco showing alteration in transgenic lines compared to the untransformed control, analysis of transgenic rice seed fatty acid profiles, overview
Brassica juncea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane domains analysis
Brassica juncea
16020
-
microsome
-
Brassica juncea
-
-
Organism
Organism
UniProt
Commentary
Textmining
Brassica juncea
E0A920
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Brassica juncea
-
Subunits
Subunits
Commentary
Organism
?
x * 43000, about, sequence calculation
Brassica juncea
Synonyms
Synonyms
Commentary
Organism
BjFad3
-
Brassica juncea
FAD3
-
Brassica juncea
omega-3 desaturase
-
Brassica juncea
Cloned(Commentary) (protein specific)
Commentary
Organism
gene fad3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant plamid pCAM::Nap-BjFad3-NOS is introduced into Agrobacterium tumefaciens strain LBA4404/VirGN54D by freeze-thaw method and then used for transformation of Escherichia coli strain DH10B and of Nicotiana tabacum explant, recombinant ectopic expression analysis in leaves of transgenic plants, recombinant expression of the enzyme in transgenic Oryza sativa plants, semi-quantitative RT-PCR expression analysis
Brassica juncea
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
seed fatty acid profiles of tobacco showing alteration in transgenic lines compared to the untransformed control, analysis of transgenic rice seed fatty acid profiles, overview
Brassica juncea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane domains analysis
Brassica juncea
16020
-
microsome
-
Brassica juncea
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Brassica juncea
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43000, about, sequence calculation
Brassica juncea
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743983
Lau
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Effects of disrupted omega-3 ...
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26
143-152
2017
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745935
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An OMEGA-3 fatty acid desatur ...
Saprolegnia diclina
Oncol. Lett.
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3717-3721
2017
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Lee
Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
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Singer
Development and characterizati ...
Brassica oleracea
BMC Genet.
15
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2014
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Radovanovic
Functional characterization of ...
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Wang
A tomato endoplasmic reticulu ...
Solanum lycopersicum
Plant Cell Rep.
33
131-142
2014
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746063
Bhattacharya
-
Heterologous expression of Br ...
Brassica juncea
Plant Cell Tissue Organ. Cult.
119
117-129
2014
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733684
Bilyeu
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Novel FAD3 mutant allele combi ...
Glycine max
Crop Sci.
51
259-264
2011
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Linum usitatissimum
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2011
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735249
Zhang
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Heterologous expression of two ...
Glycine max
Russ. J. Plant Physiol.
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569-574
2009
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731191
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A novel fungal omega3-desatura ...
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2005
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734367
Savile
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Cryptoregiochemistry of a Bras ...
Brassica napus
J. Chem. Soc. Perkin Trans. I
2001
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2001
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734987
Matsuda
Hormonal regulation of tissue- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
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2001
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733925
Hamada
Cloning of a cDNA encoding tob ...
Nicotiana tabacum
Gene
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293-294
1994
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Arabidopsis thaliana
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732914
Arondel
Map-based cloning of a gene co ...
Brassica napus
Science
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1353-1355
1992
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