BRENDA - Enzyme Database
show all sequences of 1.14.19.25

Functional identification of oleate 12-desaturase and omega-3 fatty acid desaturase genes from Perilla frutescens var. frutescens

Lee, K.R.; Lee, Y.; Kim, E.H.; Lee, S.B.; Roh, K.H.; Kim, J.B.; Kang, H.C.; Kim, H.U.; Plant Cell Rep. 35, 2523-2537 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene fad3-1, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1; gene fad3-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
Perilla frutescens var. frutescens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Perilla frutescens var. frutescens
9507
-
endoplasmic reticulum membrane
-
Perilla frutescens var. frutescens
5789
-
additional information
subcellular localization analysis, overview; subcellular localization analysis, overview
Perilla frutescens var. frutescens
-
-
Organism
Organism
UniProt
Commentary
Textmining
Perilla frutescens var. frutescens
Q8VWX1
FAD3-2; cv. Dayudeulkkae
-
Perilla frutescens var. frutescens
Q9ZPP7
FAD3-1 or FAD3b; cv. Dayudeulkkae
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Perilla frutescens var. frutescens
-
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview; expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview. No expression in stem, root, and flower
Perilla frutescens var. frutescens
-
seed
high activity and/or abundant expression in developing seeds; high activity and/or abundant expression in developing seeds
Perilla frutescens var. frutescens
-
seedling
;
Perilla frutescens var. frutescens
-
Synonyms
Synonyms
Commentary
Organism
ER-localized omega-3 FA desaturase
-
Perilla frutescens var. frutescens
FAD3
-
Perilla frutescens var. frutescens
FAD3-1
-
Perilla frutescens var. frutescens
FAD3-2
-
Perilla frutescens var. frutescens
omega-3 FA desaturase
-
Perilla frutescens var. frutescens
omega-3 fatty acid desaturase
-
Perilla frutescens var. frutescens
PfrFAD3
-
Perilla frutescens var. frutescens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene fad3-1, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
Perilla frutescens var. frutescens
gene fad3-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
Perilla frutescens var. frutescens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Perilla frutescens var. frutescens
9507
-
endoplasmic reticulum membrane
-
Perilla frutescens var. frutescens
5789
-
additional information
subcellular localization analysis, overview
Perilla frutescens var. frutescens
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Perilla frutescens var. frutescens
-
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview
Perilla frutescens var. frutescens
-
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview. No expression in stem, root, and flower
Perilla frutescens var. frutescens
-
seed
high activity and/or abundant expression in developing seeds
Perilla frutescens var. frutescens
-
seedling
-
Perilla frutescens var. frutescens
-
General Information
General Information
Commentary
Organism
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases; the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
Perilla frutescens var. frutescens
General Information (protein specific)
General Information
Commentary
Organism
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
Perilla frutescens var. frutescens
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743983
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Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
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2016
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