BRENDA - Enzyme Database
show all sequences of 1.14.19.25

An OMEGA-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation

Pan, J.; Zhou, S.; Xiang, R.; Zhao, Z.; Liu, S.; Ding, N.; Gong, S.; Lin, Y.; Li, X.; Bai, X.; Li, F.; Zhao, A.Z.; Oncol. Lett. 13, 3717-3721 (2017)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
consuming high levels of long-chain OMEGA-3 polyunsaturated fatty acids (PUFAs) is been shown to reduce prostate cancer risk and increase chemotherapy sensitivity in humans
Saprolegnia diclina
Cloned(Commentary)
Cloned (Commentary)
Organism
gene sdd17, recombinant exression of codon-otimized gene in human VCaP prostate cancer cells by lentiviral tranfection using the the PLJM1 lentivirus vector, which allows the endogenous production of OMEGA-3 polyunsaturated fatty acids (PUFAs), quantitative RT-PCR expression analysis
Saprolegnia diclina
Organism
Organism
UniProt
Commentary
Textmining
Saprolegnia diclina
Q6UB73
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
745935
Saprolegnia diclina
eicosapentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
msdd17
-
Saprolegnia diclina
omega-3 fatty acid desaturase
-
Saprolegnia diclina
sdd17
-
Saprolegnia diclina
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Saprolegnia diclina
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saprolegnia diclina
Application (protein specific)
Application
Commentary
Organism
medicine
consuming high levels of long-chain OMEGA-3 polyunsaturated fatty acids (PUFAs) is been shown to reduce prostate cancer risk and increase chemotherapy sensitivity in humans
Saprolegnia diclina
Cloned(Commentary) (protein specific)
Commentary
Organism
gene sdd17, recombinant exression of codon-otimized gene in human VCaP prostate cancer cells by lentiviral tranfection using the the PLJM1 lentivirus vector, which allows the endogenous production of OMEGA-3 polyunsaturated fatty acids (PUFAs), quantitative RT-PCR expression analysis
Saprolegnia diclina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
745935
Saprolegnia diclina
eicosapentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Saprolegnia diclina
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saprolegnia diclina
General Information
General Information
Commentary
Organism
physiological function
OMEGA-3 fatty acid desaturase converts exogenous arachidonic acid, an OMEGA-6 PUFA, into eicosapentaenoic acid (EPA). Gene sdd17 from EPA-rich fungus is expressed at high levels and increases OMEGA-3 fatty acid concentrations in mammalian cells
Saprolegnia diclina
General Information (protein specific)
General Information
Commentary
Organism
physiological function
OMEGA-3 fatty acid desaturase converts exogenous arachidonic acid, an OMEGA-6 PUFA, into eicosapentaenoic acid (EPA). Gene sdd17 from EPA-rich fungus is expressed at high levels and increases OMEGA-3 fatty acid concentrations in mammalian cells
Saprolegnia diclina
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743983
Lau
-
Effects of disrupted omega-3 ...
Chlorella vulgaris, Chlorella vulgaris UMT-M1
Algal Res.
26
143-152
2017
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
745935
Pan
An OMEGA-3 fatty acid desatur ...
Saprolegnia diclina
Oncol. Lett.
13
3717-3721
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
746058
Lee
Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
-
-
1
-
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
733575
Singer
Development and characterizati ...
Brassica oleracea
BMC Genet.
15
94
2014
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734670
Radovanovic
Functional characterization of ...
Linum usitatissimum
Mol. Biotechnol.
56
609-620
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746051
Wang
A tomato endoplasmic reticulu ...
Solanum lycopersicum
Plant Cell Rep.
33
131-142
2014
-
-
1
-
1
-
-
-
2
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
746063
Bhattacharya
-
Heterologous expression of Br ...
Brassica juncea
Plant Cell Tissue Organ. Cult.
119
117-129
2014
-
-
1
-
1
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733684
Bilyeu
-
Novel FAD3 mutant allele combi ...
Glycine max
Crop Sci.
51
259-264
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734566
Khadake
Functional and bioinformatic c ...
Linum usitatissimum
J. Sci. Food Agric.
91
2689-2696
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735249
Zhang
-
Heterologous expression of two ...
Glycine max
Russ. J. Plant Physiol.
56
569-574
2009
-
-
1
-
-
-
-
-
1
-
-
3
-
3
-
-
-
-
-
1
-
-
3
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731191
Sakuradani
A novel fungal omega3-desatura ...
Mortierella alpina, Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734367
Savile
-
Cryptoregiochemistry of a Bras ...
Brassica napus
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734987
Matsuda
Hormonal regulation of tissue- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
213
833-840
2001
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
733925
Hamada
Cloning of a cDNA encoding tob ...
Nicotiana tabacum
Gene
147
293-294
1994
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732019
Browse
Mutants of Arabidopsis deficie ...
Arabidopsis thaliana
J. Biol. Chem.
268
16345-16351
1993
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732914
Arondel
Map-based cloning of a gene co ...
Brassica napus
Science
258
1353-1355
1992
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-