BRENDA - Enzyme Database show
show all sequences of 1.14.19.25

Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae

Zhang, H.; Bi, Y.; Liu, Z.; Shan, L.; Russ. J. Plant Physiol. 56, 569-574 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain K601; expressed in Saccharomyces cerevisiae strain K601; expressed in Saccharomyces cerevisiae strain K601
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Glycine max
-
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
Q84X83
isoform FAD3B; cultivar Qihuang 29
-
Glycine max
Q84X84
isoform FAD3A; cultivar Qihuang 29
-
Glycine max
Q9FXK9
isoform FAD3C; cultivar Qihuang 29
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Glycine max
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
735249
Glycine max
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain K601
Glycine max
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Glycine max
-
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
735249
Glycine max
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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733575
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Brassica oleracea
BMC Genet.
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2014
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1
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734670
Radovanovic
Functional characterization of ...
Linum usitatissimum
Mol. Biotechnol.
56
609-620
2014
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1
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746051
Wang
A tomato endoplasmic reticulu ...
Solanum lycopersicum
Plant Cell Rep.
33
131-142
2014
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1
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1
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746063
Bhattacharya
-
Heterologous expression of Br ...
Brassica juncea
Plant Cell Tissue Organ. Cult.
119
117-129
2014
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1
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1
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2
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733684
Bilyeu
-
Novel FAD3 mutant allele combi ...
Glycine max
Crop Sci.
51
259-264
2011
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734566
Khadake
Functional and bioinformatic c ...
Linum usitatissimum
J. Sci. Food Agric.
91
2689-2696
2011
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1
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735249
Zhang
-
Heterologous expression of two ...
Glycine max
Russ. J. Plant Physiol.
56
569-574
2009
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1
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1
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3
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1
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731191
Sakuradani
A novel fungal omega3-desatura ...
Mortierella alpina, Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
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734367
Savile
-
Cryptoregiochemistry of a Bras ...
Brassica napus
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
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734987
Matsuda
Hormonal regulation of tissue- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
213
833-840
2001
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1
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733925
Hamada
Cloning of a cDNA encoding tob ...
Nicotiana tabacum
Gene
147
293-294
1994
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732019
Browse
Mutants of Arabidopsis deficie ...
Arabidopsis thaliana
J. Biol. Chem.
268
16345-16351
1993
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732914
Arondel
Map-based cloning of a gene co ...
Brassica napus
Science
258
1353-1355
1992
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