BRENDA - Enzyme Database
show all sequences of 1.14.19.25

Development and characterization of low alpha-linolenic acid Brassica oleracea lines bearing a novel mutation in a class a FATTY ACID DESATURASE 3 gene

Singer, S.; Weselake, R.; Rahman, H.; BMC Genet. 15, 94 (2014)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
a mutant protein lacking 110 amino acids at its C terminus exhibits a drastic decline in its DELTA-15 desaturase activity compared to the wild type protein
Brassica oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Brassica oleracea
-
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica oleracea
-
Brassica oleracea var. alboglabra
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Brassica oleracea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
733575
Brassica oleracea
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Brassica oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b5
-
Brassica oleracea
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a mutant protein lacking 110 amino acids at its C terminus exhibits a drastic decline in its DELTA-15 desaturase activity compared to the wild type protein
Brassica oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
Brassica oleracea
-
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Brassica oleracea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
733575
Brassica oleracea
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743983
Lau
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143-152
2017
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745935
Pan
An OMEGA-3 fatty acid desatur ...
Saprolegnia diclina
Oncol. Lett.
13
3717-3721
2017
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746058
Lee
Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
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733575
Singer
Development and characterizati ...
Brassica oleracea
BMC Genet.
15
94
2014
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734670
Radovanovic
Functional characterization of ...
Linum usitatissimum
Mol. Biotechnol.
56
609-620
2014
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746051
Wang
A tomato endoplasmic reticulu ...
Solanum lycopersicum
Plant Cell Rep.
33
131-142
2014
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746063
Bhattacharya
-
Heterologous expression of Br ...
Brassica juncea
Plant Cell Tissue Organ. Cult.
119
117-129
2014
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733684
Bilyeu
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Novel FAD3 mutant allele combi ...
Glycine max
Crop Sci.
51
259-264
2011
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734566
Khadake
Functional and bioinformatic c ...
Linum usitatissimum
J. Sci. Food Agric.
91
2689-2696
2011
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735249
Zhang
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Heterologous expression of two ...
Glycine max
Russ. J. Plant Physiol.
56
569-574
2009
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1
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3
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731191
Sakuradani
A novel fungal omega3-desatura ...
Mortierella alpina, Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
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734367
Savile
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Cryptoregiochemistry of a Bras ...
Brassica napus
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
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734987
Matsuda
Hormonal regulation of tissue- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
213
833-840
2001
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733925
Hamada
Cloning of a cDNA encoding tob ...
Nicotiana tabacum
Gene
147
293-294
1994
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Browse
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Arabidopsis thaliana
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1993
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732914
Arondel
Map-based cloning of a gene co ...
Brassica napus
Science
258
1353-1355
1992
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