BRENDA - Enzyme Database
show all sequences of 1.14.19.25

A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4

Sakuradani, E.; Abe, T.; Iguchi, K.; Shimizu, S.; Appl. Microbiol. Biotechnol. 66, 648-654 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae
Mortierella alpina
Organism
Organism
UniProt
Commentary
Textmining
Mortierella alpina
Q59J82
-
-
Mortierella alpina 1S-4
Q59J82
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
731191
Mortierella alpina
?
-
-
-
-
additional information
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
731191
Mortierella alpina 1S-4
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
maw3
-
Mortierella alpina
omefa3-desaturase
ambiguous
Mortierella alpina
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Mortierella alpina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
731191
Mortierella alpina 1S-4
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
additional information
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
731191
Mortierella alpina
?
-
-
-
-
additional information
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
731191
Mortierella alpina 1S-4
?
-
-
-
-
Other publictions for EC 1.14.19.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743983
Lau
-
Effects of disrupted omega-3 ...
Chlorella vulgaris, Chlorella vulgaris UMT-M1
Algal Res.
26
143-152
2017
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745935
Pan
An OMEGA-3 fatty acid desatur ...
Saprolegnia diclina
Oncol. Lett.
13
3717-3721
2017
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746058
Lee
Functional identification of ...
Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
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733575
Singer
Development and characterizati ...
Brassica oleracea
BMC Genet.
15
94
2014
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734670
Radovanovic
Functional characterization of ...
Linum usitatissimum
Mol. Biotechnol.
56
609-620
2014
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746051
Wang
A tomato endoplasmic reticulu ...
Solanum lycopersicum
Plant Cell Rep.
33
131-142
2014
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746063
Bhattacharya
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Heterologous expression of Br ...
Brassica juncea
Plant Cell Tissue Organ. Cult.
119
117-129
2014
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733684
Bilyeu
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Novel FAD3 mutant allele combi ...
Glycine max
Crop Sci.
51
259-264
2011
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734566
Khadake
Functional and bioinformatic c ...
Linum usitatissimum
J. Sci. Food Agric.
91
2689-2696
2011
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735249
Zhang
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Heterologous expression of two ...
Glycine max
Russ. J. Plant Physiol.
56
569-574
2009
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731191
Sakuradani
A novel fungal omega3-desatura ...
Mortierella alpina, Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
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734367
Savile
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Cryptoregiochemistry of a Bras ...
Brassica napus
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
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734987
Matsuda
Hormonal regulation of tissue- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Planta
213
833-840
2001
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