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Literature summary for 1.14.19.22 extracted from

  • Broadwater, J.A.; Whittle, E.; Shanklin, J.
    Desaturation and hydroxylation. Residues 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity (2002), J. Biol. Chem., 277, 15613-15620.
    View publication on PubMed
Search for more literature for 1.14.19.22

Protein Variants

Protein Variants Comment Organism
A104G 2.1fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/M324V 2.6fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/S322A 3.1fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/S322A/M324V 4.3fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/T148I 29.5fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/T148I/M324V 36.1fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/T148I/S322A 44.3fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/T148I/S322A/M324V 90.2fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
A104G/T148N/S322A/M324I 24.6fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
M324I 54.1fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
M324V 49.2fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
S322A 1.3fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
S322A/M324V 2.1fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
T148I 14.8fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
T148I/M324V 26.2fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
T148I/S322A 26.2fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
T148I/S322A/M324V 34.4fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana
T148N 3.3fold increase in the ratio of hydroxylation to desaturation compared to wild-type enzyme Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information wild-type enzyme has an inherent, low level of hydroxylation activity Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
oleate desaturase
-
 Arabidopsis thaliana