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Literature summary for 1.14.19.20 extracted from
- Partial purification and characterization of lathosterol 5-desaturase from rat liver microsomes (1985), J. Biochem., 97, 955-959.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | complete inhibition at 5 mM | Rattus norvegicus |  |
| additional information | the enzyme is not affected by N-ethylmaleimide and dithiothreitol | Rattus norvegicus | |
| Tiron | 21% residual activity at 1 mM | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lathosterol + ferrocytochrome b5 + O2 + H+ | Rattus norvegicus | - |
7-dehydrocholesterol + ferricytochrome b5 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification by DEAE cellulose column chromatography and 6-amino-hexyl-Sepharose column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0013 | - |
crude enzyme, at pH 8.0 and 37°C | Rattus norvegicus |
| 0.022 | - |
after 17.7fold purification, at pH 8.0 and 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+ | - |
Rattus norvegicus | ? + ferricytochrome b5 + H2O | - |
? | |
| lathosterol + ferrocytochrome b5 + O2 + H+ | - |
Rattus norvegicus | 7-dehydrocholesterol + ferricytochrome b5 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lathosterol 5-desaturase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | dependent on | Rattus norvegicus | |
| NADH | dependent on | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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