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Literature summary for 1.14.19.20 extracted from
- Plant sterol biosynthesis: Identification and characterization of higher plant DELTA7-sterol C5(6)-desaturase (1996), Arch. Biochem. Biophys., 325, 279-288.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Zea mays |  |
| cyanide | - |
Zea mays | |
| CYC (cyctochrome c) | - |
Zea mays | |
| menadione | 32% residual actitivty at 0.05 mM | Zea mays | |
| additional information | not inhibited by CO | Zea mays | |
| Salicylhydroxamic acid | - |
Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0105 | - |
episterol | at pH 7.6 and 30°C | Zea mays | |
| 0.08 | - |
stigmasta-7,22-dien-3beta-ol | at pH 7.6 and 30°C | Zea mays | |
| 0.115 | - |
lathosterol | at pH 7.6 and 30°C | Zea mays | |
| 0.14 | - |
avenasterol | at pH 7.6 and 30°C | Zea mays | |
| 0.67 | - |
stigmast-7-en-3beta-ol | at pH 7.6 and 30°C | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Zea mays | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DELTA7-sterol + ferrocytochrome b5 + O2 + H+ | Zea mays | - |
DELTA5,7-sterol + ferricytochrome b5 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avenasterol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | ? | - |
? | |
| DELTA7-sterol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | DELTA5,7-sterol + ferricytochrome b5 + H2O | - |
? | |
| episterol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | ergosta-5,7,24(28)-trien-3beta-ol + ferricytochrome b5 + 2 H2O | - |
? | |
| lathosterol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | ? | - |
? | |
| additional information | no activity with 4alpha-methyl-5alpha-cholest-7-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-cholest-7-en-3beta-ol, cholestan-3beta-ol and cholest-8-en-3beta-ol | Zea mays | ? | - |
? | |
| additional information | the specificity of maize C5(6)-desaturase favors DELTA7-sterols possessing a C24-methylene or ethylidene substituent compared to 24-ethyl-substituted DELTA7-sterols | Zea mays | ? | - |
? | |
| stigmast-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | stigmast-5,7-dien-3beta-ol + ferricyctochrome b5 + H2O | - |
? | |
| stigmasta-7,22-dien-3beta-ol + ferrocytochrome b5 + O2 + H+ | - |
Zea mays | stigmast-5,7,22-trien-3beta-ol + ferricyctochrome b5 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA7-sterol C56-desaturase | - |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | - |
Zea mays | |
| NADH | required, NADH is more efficient than NADPH | Zea mays | |
| NADPH | NADH is more efficient than NADPH | Zea mays | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
at pH 7.6 and 30°C | Zea mays | CYC (cyctochrome c) | |
| 0.015 | - |
at pH 7.6 and 30°C | Zea mays | cyanide | |
| 0.5 | - |
at pH 7.6 and 30°C | Zea mays | Salicylhydroxamic acid | |
| 2 | - |
at pH 7.6 and 30°C | Zea mays | 1,10-phenanthroline | |
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